LOCUS       sequence-134131-        9404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human PIK3CA E545K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Baek ST, Copeland B, Yun EJ, Kwon SK, Guemez-Gamboa A, Schaffer AE, 
            Kim S, Kang HC, Song S, Mathern GW, Gleeson JG
  TITLE     An AKT3-FOXG1-reelin network underlies defective migration in human 
            focal malformations of cortical development.
  JOURNAL   Nat Med. 2015 Dec;21(12):1445-54. doi: 10.1038/nm.3982. Epub 2015 
            Nov 2.
  PUBMED    26523971
REFERENCE   2  (bases 1 to 9404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nm.3982"; 
            journalName: "Nat Med"; date: "2015-12"; volume: "21"; issue: "12"; 
            pages: "1445-54"
COMMENT     Sequence Label: pCIG PIK3CA E545K
FEATURES             Location/Qualifiers
     source          1..9404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(122..141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(801..1592,1593..1661))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1424..1443
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1662..1766)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1797..2176
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2178..2455
                     /label=chicken beta-actin promoter
     intron          2456..3468
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3392..3414
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3476..3495
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    join(6790..7364,7365..7367,7368..7376)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6957..6974)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7184..7203
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7377..7379,7380..7382,7383..8096)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7377..7379,7380..7382,7383..8093)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7422..7443)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7683..7702)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8030..8051
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8188..8207)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8253..8308
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8307..8326)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8699..8715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8699..8715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8712..8734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8723..8739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8747..8753,8754..8771,8772..8777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8792..8813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8872..9067
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8918..9053
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9073..9207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9110..9129)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9164..9183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9275..9292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 agnacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      661 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      721 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      781 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      841 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      901 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      961 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1021 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1081 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1141 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1201 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1261 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1321 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1381 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1441 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1501 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1561 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1621 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1681 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1741 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca
     1801 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1861 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     1921 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1981 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2041 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2101 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2161 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     2221 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     2281 ggcggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
     2341 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
     2401 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
     2461 cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct
     2521 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
     2581 taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa
     2641 agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg
     2701 tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg
     2761 cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc
     2821 ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     2881 ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc
     2941 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     3001 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     3061 cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga
     3121 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
     3181 cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
     3241 gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     3301 gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac
     3361 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     3421 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     3481 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgctcgagct catgcctcca
     3541 cgaccatcat caggtgaact gtggggcatc cacttgatgc ccccaagaat cctagtagaa
     3601 tgtttactac caaatggaat gatagtgact ttagaatgcc tccgtgaggc tacattaata
     3661 accataaagc atgaactatt taaagaagca agaaaatacc ccctccatca acttcttcaa
     3721 gatgaatctt cttacatttt cgtaagtgtt actcaagaag cagaaaggga agaatttttt
     3781 gatgaaacaa gacgactttg tgaccttcgg ctttttcaac cctttttaaa agtaattgaa
     3841 ccagtaggca accgtgaaga aaagatcctc aatcgagaaa ttggttttgc tatcggcatg
     3901 ccagtgtgtg aatttgatat ggttaaagat ccagaagtac aggacttccg aagaaatatt
     3961 ctgaacgttt gtaaagaagc tgtggatctt agggacctca attcacctca tagtagagca
     4021 atgtatgtct atcctccaaa tgtagaatct tcaccagaat tgccaaagca catatataat
     4081 aaattagata aagggcaaat aatagtggtg atctgggtaa tagtttctcc aaataatgac
     4141 aagcagaagt atactctgaa aatcaaccat gactgtgtac cagaacaagt aattgctgaa
     4201 gcaatcagga aaaaaactcg aagtatgttg ctatcctctg aacaactaaa actctgtgtt
     4261 ttagaatatc agggcaagta tattttaaaa gtgtgtggat gtgatgaata cttcctagaa
     4321 aaatatcctc tgagtcagta taagtatata agaagctgta taatgcttgg gaggatgccc
     4381 aatttgatgt tgatggctaa agaaagcctt tattctcaac tgccaatgga ctgttttaca
     4441 atgccatctt attccagacg catttccaca gctacaccat atatgaatgg agaaacatct
     4501 acaaaatccc tttgggttat aaatagtgca ctcagaataa aaattctttg tgcaacctac
     4561 gtgaatgtaa atattcgaga cattgataag atctatgttc gaacaggtat ctaccatgga
     4621 ggagaaccct tatgtgacaa tgtgaacact caaagagtac cttgttccaa tcccaggtgg
     4681 aatgaatggc tgaattatga tatatacatt cctgatcttc ctcgtgctgc tcgactttgc
     4741 ctttccattt gctctgttaa aggccgaaag ggtgctaaag aggaacactg tccattggca
     4801 tggggaaata taaacttgtt tgattacaca gacactctag tatctggaaa aatggctttg
     4861 aatctttggc cagtacctca tggattagaa gatttgctga accctattgg tgttactgga
     4921 tcaaatccaa ataaagaaac tccatgctta gagttggagt ttgactggtt cagcagtgtg
     4981 gtaaagttcc cagatatgtc agtgattgaa gagcatgcca attggtctgt atcccgagaa
     5041 gcaggattta gctattccca cgcaggactg agtaacagac tagctagaga caatgaatta
     5101 agggaaaatg acaaagaaca gctcaaagca atttctacac gagatcctct ctctgaaatc
     5161 actaagcagg agaaagattt tctatggagt cacagacact attgtgtaac tatccccgaa
     5221 attctaccca aattgcttct gtctgttaaa tggaattcta gagatgaagt agcccagatg
     5281 tattgcttgg taaaagattg gcctccaatc aaacctgaac aggctatgga acttctggac
     5341 tgtaattacc cagatcctat ggttcgaggt tttgctgttc ggtgcttgga aaaatattta
     5401 acagatgaca aactttctca gtatttaatt cagctagtac aggtcctaaa atatgaacaa
     5461 tatttggata acttgcttgt gagattttta ctgaagaaag cattgactaa tcaaaggatt
     5521 gggcactttt tcttttggca tttaaaatct gagatgcaca ataaaacagt tagccagagg
     5581 tttggcctgc ttttggagtc ctattgtcgt gcatgtggga tgtatttgaa gcacctgaat
     5641 aggcaagtcg aggcaatgga aaagctcatt aacttaactg acattctcaa acaggagaag
     5701 aaggatgaaa cacaaaaggt acagatgaag tttttagttg agcaaatgag gcgaccagat
     5761 ttcatggatg ctctacaggg ctttctgtct cctctaaacc ctgctcatca actaggaaac
     5821 ctcaggcttg aagagtgtcg aattatgtcc tctgcaaaaa ggccactgtg gttgaattgg
     5881 gagaacccag acatcatgtc agagttactg tttcagaaca atgagatcat ctttaaaaat
     5941 ggggatgatt tacggcaaga tatgctaaca cttcaaatta ttcgtattat ggaaaatatc
     6001 tggcaaaatc aaggtcttga tcttcgaatg ttaccttatg gttgtctgtc aatcggtgac
     6061 tgtgtgggac ttattgaggt ggtgcgaaat tctcacacta ttatgcaaat tcagtgcaaa
     6121 ggcggcttga aaggtgcact gcagttcaac agccacacac tacatcagtg gctcaaagac
     6181 aagaacaaag gagaaatata tgatgcagcc attgacctgt ttacacgttc atgtgctgga
     6241 tactgtgtag ctaccttcat tttgggaatt ggagatcgtc acaatagtaa catcatggtg
     6301 aaagacgatg gacaactgtt tcatatagat tttggacact ttttggatca caagaagaaa
     6361 aaatttggtt ataaacgaga acgtgtgcca tttgttttga cacaggattt cttaatagtg
     6421 attagtaaag gagcccaaga atgcacaaag acaagagaat ttgagaggtt tcaggagatg
     6481 tgttacaagg cttatctagc tattcgacag catgccaatc tcttcataaa tcttttctca
     6541 atgatgcttg gctctggaat gccagaacta caatcttttg atgacattgc atacattcga
     6601 aagaccctag ccttagataa aactgagcaa gaggctttgg agtatttcat gaaacaaatg
     6661 aatgatgcac atcatggtgg ctggacaaca aaaatggatt ggatcttcca cacaattaaa
     6721 cagcatgcat tgaactgacc tgcaggctag cgaattctgc agtcgacggt accgcgggcc
     6781 cgggatccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     6841 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     6901 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     6961 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     7021 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     7081 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     7141 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     7201 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     7261 gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     7321 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg
     7381 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     7441 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     7501 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     7561 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     7621 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     7681 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     7741 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     7801 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     7861 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     7921 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     7981 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     8041 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg
     8101 gccgcaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     8161 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     8221 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     8281 agtgtgttgg aattttttgt gtctatcact cggaaggaca tatgggaggg caaatcattt
     8341 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     8401 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     8461 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     8521 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     8581 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     8641 cctgcaccgt cgaccagctg gtcgacggtg caccgtcgac cagcttggcg taatcatggt
     8701 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8761 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8821 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa
     8881 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8941 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9001 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9061 ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9121 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9181 atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga
     9241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccag
//
