LOCUS       sequence-134133-        6965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal GFP fusion of Mouse Foxg1 T271E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Baek ST, Copeland B, Yun EJ, Kwon SK, Guemez-Gamboa A, Schaffer AE, 
            Kim S, Kang HC, Song S, Mathern GW, Gleeson JG
  TITLE     An AKT3-FOXG1-reelin network underlies defective migration in human 
            focal malformations of cortical development.
  JOURNAL   Nat Med. 2015 Dec;21(12):1445-54. doi: 10.1038/nm.3982. Epub 2015 
            Nov 2.
  PUBMED    26523971
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nm.3982"; 
            journalName: "Nat Med"; date: "2015-12"; volume: "21"; issue: "12"; 
            pages: "1445-54"
COMMENT     Sequence Label: pCAG mFoxg1-GFP T271E
FEATURES             Location/Qualifiers
     source          1..6965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(42..630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(122..141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(801..1592,1593..1661))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1424..1443
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1662..1766)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1796..2175
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2178..2453
                     /label=chicken beta-actin promoter
     intron          2454..3462
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3386..3408
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3470..3489
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3655..3684
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     regulatory      4969..4978
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4975..4977,4978..4980,4981..5694)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4975..4977,4978..4980,4981..5691)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5020..5041)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5281..5300)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5628..5649
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5781..5800)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5846..5901
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5900..5919)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6260..6276)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6260..6276)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6273..6295)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6284..6300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6308..6314,6315..6332,6333..6338))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6353..6374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6433..6628
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6479..6614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6634..6768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6671..6690)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6725..6744
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6836..6853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
       61 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
      241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      481 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      661 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      721 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      781 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      841 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      901 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      961 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1021 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1081 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1141 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1201 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1261 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1321 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1381 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1441 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1501 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1561 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1621 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1681 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1741 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tggtcgacat
     1801 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1861 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1921 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1981 cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg
     2041 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2101 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2161 atcgctatta ccatgggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     2221 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     2281 ggcggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
     2341 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     2401 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     2461 ctgcgttgcc ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg ccccggctct
     2521 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
     2581 attagcgctt ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa agccttaaag
     2641 ggctccggga gggccctttg tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     2701 tgcgtgggga gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
     2761 gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc
     2821 gcggtgcggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     2881 tgagcagggg gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg
     2941 agttgctgag cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct
     3001 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
     3061 ggccggggag ggctcggggg aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc
     3121 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     3181 ttgtcccaaa tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     3241 gcgggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     3301 gccgcgccgc cgtccccttc tccatctcca gcctcggggc tgccgcaggg ggacggctgc
     3361 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     3421 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     3481 gttattgtgc tgtctcatca ttttggcaaa gaattcatgc tggacatggg agataggaaa
     3541 gaggtgaaaa tgattcccaa gtcctcgttc agcatcaaca gcctggtccc cgaggccgtc
     3601 cagaacgaca accaccacgc gagccacggc caccacaaca gccaccaccc ccagcatcac
     3661 catcatcatc accaccacca ccacccgccg ccgcccgcgc cccagccgcc tccaccgccg
     3721 ccccagcagc agcagcagca gccgcccccg gccccgcagc ccccgcaggc gcgcggcgcc
     3781 ccagcagccg acgacgacaa gggtccccag ccgctcctgc tcccgccctc caccgccctg
     3841 gacggggcca aggctgacgc acttggagcc aaaggcgagc cgggcggcgg gccggcggag
     3901 ctggcgcccg tcgggccgga cgagaaggag aagggcgcgg gcgccggggg ggaggagaag
     3961 aaaggggcgg gcgagggcgg caaggacggg gaggggggca aggagggcga caagaagaac
     4021 ggcaagtacg agaagccgcc gttcagctac aacgcgctca tcatgatggc catcaggcag
     4081 agtcccgaga agcgcctgac gctcaatggc atctatgagt tcatcatgaa gaacttcccc
     4141 tactaccgcg agaacaagca gggctggcag aactccatcc gccacaacct gtccctcaac
     4201 aagtgcttcg tgaaggtacc gcgccactac gacgacccgg gcaagggcaa ctactggatg
     4261 ctcgacccgt cgagcgacga cgtgttcatc ggcggcacga ccggcaagct gcggcgccgc
     4321 tccaccgagt ctcgggccaa gctggccttt aagcgcgggg cgcgcctcac ctccaccggc
     4381 ctcaccttca tggaccgcgc cggctccctc tactggccca tgtcgccctt cctgtccctg
     4441 caccaccccc gcgccagcag cactttgagt tacaacggga ccacgtcggc ctaccccagc
     4501 caccccatgc cctacagctc cgtgttgact caaaactcgc tgggcaacaa ccactccttc
     4561 tccaccgcca acgggctgag tgtggaccgg ctggtcaacg gggagatccc gtacgccacg
     4621 caccacctca cggccgctgc gctcgccgcc tcggtgccct gcggcctgtc ggtgccctgc
     4681 tccgggacct actccctcaa cccctgctcc gtcaacctgc tcgcgggcca gaccagttac
     4741 tttttccccc acgtcccgca cccgtcaatg acttcgcaga ccagcacgtc catgagcgcc
     4801 cgggccgcgt cctcctctac gtcgccgcag gccccctcga ccctgccctg tgagtcttta
     4861 agaccctctt tgccaagttt tacgacagga ctgtccgggg gactgtctga ttatttcaca
     4921 catcaaaatc aggggtcttc ttccaaccct ttaatacatg gaccggtcgc caccatggtg
     4981 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     5041 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     5101 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     5161 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     5221 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     5281 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     5341 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     5401 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     5461 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     5521 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5581 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5641 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     5701 cgcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     5761 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     5821 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     5881 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     5941 cagaatgagt atttggttta gagtttggca acatatgcca tatgctggct gccatgaaca
     6001 aaggtggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     6061 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     6121 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     6181 ctgactactc ccagtcatag ctgtccctct tctcttatga agatccctcg acctgcagcc
     6241 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6301 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6361 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6421 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6481 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6541 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6601 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt
     6661 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6721 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat
     6781 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6841 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6901 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6961 gccag
//
