LOCUS       sequence-134137-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloned region contains Acetobacter aceti 1023 purE(H59D) gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK346
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             569..577
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(593..610)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(593..609)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(602..624)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aaaccggata gatacccacc gtgagcgaaa ccgcccccct gccctctgct tccagtgctt
       61 tagaagataa agcagcctct gcccccgttg tgggcattat catgggcagc cagtcagact
      121 gggaaaccat gcgccatgca gacgcattgc tgacagagct ggaaattccg catgaaaccc
      181 tcattgtttc tgcagatcgt acgccagacc ggttggcgga ttatgcgcgt acggcagccg
      241 aacgcggcct gaatgtgatt attgctgggg ccggtggagc tgcacatctg ccgggtatgt
      301 gtgcggcatg gacacgcctg cccgtgctgg gtgttccagt agaatctcgc gctcttaaag
      361 gcatggacag cctgctttcc attgtgcaga tgcccggcgg cgtgcctgtg ggcactttgg
      421 ctattggggc atccggcgcc aaaaatgccg ccttgctggc cgcctccatt cttgcactgt
      481 ataacccggc ccttgccgca cgccttgaaa catggcgcgc attgcagacg gcctctgtcc
      541 ccaactctcc cattaccgaa gataaatgac ctccttacat tctaagcttg gcactggccg
      601 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      661 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      721 aacagttgcg cagcctgaat ggcgaatgg
//
