LOCUS       sequence-134143-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Grashoff C, Hoffman BD, Brenner MD, Zhou R, Parsons M, Yang MT, 
            McLean MA, Sligar SG, Chen CS, Ha T, Schwartz MA
  TITLE     Measuring mechanical tension across vinculin reveals regulation of 
            focal adhesion dynamics.
  JOURNAL   Nature. 2010 Jul 8. 466(7303):263-6.
  PUBMED    20613844
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 8. 466(7303):263-6."
COMMENT     Sequence Label: TS module
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..31
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(121..138)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    127..351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(484..503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
       61 cgagctgtac aagtaggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag
      121 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      181 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      241 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
      301 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
      361 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
      421 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      481 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      541 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      601 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      661 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
//
