LOCUS       sequence-134154-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Zhao B, Li L, Lu Q, Wang LH, Liu CY, Lei Q, Guan KL
  TITLE     Angiomotin is a novel Hippo pathway component that inhibits YAP 
            oncoprotein.
  JOURNAL   Genes Dev. 2011 Jan 1;25(1):51-63.
  PUBMED    21205866
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2011-01-1"; volume: "25(1)"; pages: "51-63"
COMMENT     Sequence Label: Lenti GFP-AMOT p130 Y242/287A
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..621)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(105..125)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     602..621
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 atccagaggt tgattgtcga ctcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg
       61 tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag
      121 gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac
      181 tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac
      241 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
      301 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
      361 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc
      421 cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc
      481 ggtgacccgc tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc
      541 gaacgcggcg gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac
      601 cgtgggcttg tactcggtca tctagcgtag gcgccggtca cagcttggat ctgtaacggc
      661 gcagaacaga aaacgaaaca aagacgtaga gttgagcaag cagggtcagg caaagcgtgg
      721 agagccggct gagtctaggt aggctccaag ggagcgccgg acaaaggccc ggtctcgacc
      781 tgagctt
//
