LOCUS       sequence-134157-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid - trimethoprim resistant donor cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Tas H, Nguyen CT, Patel R, Kim NH, Kuhlman TE
  TITLE     An Integrated System for Precise Genome Modification in Escherichia 
            coli.
  JOURNAL   PLoS One. 2015 Sep 2;10(9):e0136963. doi: 
            10.1371/journal.pone.0136963. eCollection 2015.
  PUBMED    26332675
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-2"; volume: "10(9):e0136963. doi"; pages: " 
            10.1371/journal.pone.0136963. eCollection 2015"
COMMENT     Sequence Label: pTKDP-dhfr
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     89..105
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     90..106
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    128..161
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        225..724
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(260..283)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     657..676
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        736..783
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gcgcacattt ccccgaaaag tgccacctta gggataacag ggtaattacg gccccaaggt
       61 ccaaacggtg aaggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc
      121 gaattccgaa gttcctattc tctagaaagt ataggaactt caggtctgaa gaggagttta
      181 cgtccagcca agctagcttg gctgcaggtc gtcgaaattc taccgggtag gggaggcgct
      241 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
      301 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
      361 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
      421 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
      481 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
      541 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
      601 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
      661 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
      721 ttcgacctgc agcctgttga caattaatca tcggcatagt atatcggcat agtataatac
      781 gacaaggtga ggaactaaac catgggtcaa agtagcgatg aagccaacgc tcc
//
