LOCUS       sequence-134167-        2874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone containing ER-localized Venus. Can be used to construct 
            transcriptional reporters. For use in plants and compatible with the
            MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: p221z-erVenusYFP
FEATURES             Location/Qualifiers
     source          1..2874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(107..125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(130..146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(130..146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(214..588)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(607..654)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      744..1332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1233..1252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1486..1503
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1662..1689)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1781..1867)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1916..1938
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1930..1947
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1931..1947
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1963..2062
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2134..2136,2137..2847)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2176..2197)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2437..2456)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2784..2805
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(join(2864..2874,1..89))
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
       61 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
      121 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
      181 tacattgcac aagataaaat aatatcatca tgatcagtcc tgctcctcgg ccacgaagtg
      241 cacgcagttg ccggccgggt cgcgcagggc gaactcccgc ccccacggct gctcgccgat
      301 ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg gacacgacct ccgaccactc
      361 ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc agggtgttgt ccggcaccac
      421 ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc cggaccacac cggcgaagtc
      481 gtcctccacg aagtcccggg agaacccgag ccggtcggtc cagaactcga ccgctccggc
      541 gacgtcgcgc gcggtgagca ccggaacggc actggtcaac ttggccatgg tttagttcct
      601 caccttgtcg tattatacta tgccgatata ctatgccgat gattaattgt caacacgtgc
      661 tgatcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
      721 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      781 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      841 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      901 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      961 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1021 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1081 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1141 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1201 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1261 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1321 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1381 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1441 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1501 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1561 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     1621 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     1681 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     1741 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     1801 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     1861 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     1921 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
     1981 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
     2041 actttgtaca aaaaagcagg cttaagctta acaatgaaga ctaatctttt tctctttctc
     2101 atcttttcac ttctcctatc attatcctcg gccgtgagca agggcgagga gctgttcacc
     2161 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2221 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc
     2281 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag
     2341 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2401 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2461 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2521 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2581 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     2641 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2701 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     2761 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2821 actctcggca tggacgagct gtacaagcat gatgagcttt aatacccagc tttc
//
