LOCUS       sequence-134168-        2856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone containing ER-localized TagRFP. Can be used to construct
            transcriptional reporters. For use in plants and compatible with the
            MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: p221z-erTagRFP
FEATURES             Location/Qualifiers
     source          1..2856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             95..781
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALKL
                     VGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYCD
                     LPSKLGH"
     protein_bind    complement(801..900)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(917..936)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(918..936)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(941..957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(941..957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(1025..1399)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1418..1465)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      1555..2143
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2044..2063
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2297..2314
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2473..2500)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2592..2678)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2727..2749
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2741..2758
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2742..2758
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(2774..2856,1..17)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtacaaaaaa gcaggcttaa caatgaagac taatcttttt ctctttctca tcttttcact
       61 tctcctatca ttatcctcgg ccgaattcac cggtagcgag ctgattaagg agaacatgca
      121 catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg
      181 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
      241 tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca gaaccttcat
      301 caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg gcttcacatg
      361 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
      421 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc catccaacgg
      481 ccctgtgatg cagaagaaaa cactcggctg ggaggccaac accgagatgc tgtaccccgc
      541 tgacggcggc ctggaaggca gaagcgacat ggccctgaag ctcgtgggcg ggggccacct
      601 gatctgcaac ttcaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc
      661 cggcgtctac tatgtggacc acagactgga aagaatcaag gaggccgaca aagagaccta
      721 cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca aactggggca
      781 caagcatgat gagctttaac acccagcttt cttgtacaaa gttggcatta taagaaagca
      841 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
      901 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
      961 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa taatatcatc
     1021 atgatcagtc ctgctcctcg gccacgaagt gcacgcagtt gccggccggg tcgcgcaggg
     1081 cgaactcccg cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc
     1141 ggaagttcgt ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc
     1201 acacccaggc cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg
     1261 tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga
     1321 gccggtcggt ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg
     1381 cactggtcaa cttggccatg gtttagttcc tcaccttgtc gtattatact atgccgatat
     1441 actatgccga tgattaattg tcaacacgtg ctgatcatga ccaaaatccc ttaacgtgag
     1501 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1561 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1621 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1681 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1741 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1801 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1861 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1921 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1981 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2041 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2101 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2161 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2221 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2281 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2341 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2401 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
     2461 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
     2521 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
     2581 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     2641 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
     2701 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
     2761 taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
     2821 aattgatgag caatgctttt ttataatgcc aacttt
//
