LOCUS       sequence-134169-        7895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Wu Y, Borde M, Heissmeyer V, Feuerer M, Lapan AD, Stroud JC, Bates 
            DL, Guo L, Han A, Ziegler SF, Mathis D, Benoist C, Chen L, Rao A
  TITLE     FOXP3 controls regulatory T cell function through cooperation with 
            NFAT.
  JOURNAL   Cell. 2006 Jul 28. 126(2):375-87.
  PUBMED    16873067
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Jul 28. 126(2):375-87."
COMMENT     Sequence Label: MSCV-FOXP3-IRES-Thy1.1
FEATURES             Location/Qualifiers
     source          1..7895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     207..229
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     357..376
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             576..1092
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    1156..1497
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1564..1980
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1910..1932
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1948..1964
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    2029..2053
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2078..2087
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2087..2116
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3620..3636)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3621..3637)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(3647..3671)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3705..4271
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3721..4273
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3865..3882)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4092..4111
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4321..4809
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     LTR             4865..5379
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5541..5557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5565..5571,5572..5589,5590..5595))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5610..5631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5748..5765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5919..6507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5999..6018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6678..7469,7470..7538))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7301..7320
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7539..7643)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7711..7729
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7767..7789)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     join(7889..7895,1..13)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
       61 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
      121 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
      181 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg
      241 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
      301 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg
      361 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
      421 cgtccggcgt agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct
      481 ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa
      541 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
      601 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag
      661 agaagttcag atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc
      721 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
      781 ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
      841 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
      901 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc
      961 gatagactgc gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg
     1021 aatcgtggac tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg
     1081 gggtctttca tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc
     1141 cccccgccgg gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt
     1201 gtttgtgccg gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt
     1261 atctggcgga cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga
     1321 cgtcccaggg actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg
     1381 aatccgaccc cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg
     1441 cctccgtctg aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag
     1501 cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
     1561 tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag
     1621 cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct
     1681 gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc
     1741 atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc
     1801 ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt
     1861 gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct
     1921 cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc
     1981 ggaattagat ctctcgagat cgatgcggcc gcgtttaaac gtcatcaaac aagtttgtac
     2041 aaaaaagcag gcttcgaagg agatagaacc ggtggcggcc gccatggagc agaaactcat
     2101 ctctgaagag gatctgagcc cgggcggatc ccccgggctg caggaattcg gctcctggga
     2161 caaggacccg atgcccaacc ccaggcctgg caagccctcg gccccttcct tggcccttgg
     2221 cccatcccca ggagcctcgc ccagctggag ggctgcaccc aaagcctcag acctgctggg
     2281 ggcccggggc ccagggggaa ccttccaggg ccgagatctt cgaggcgggg cccatgcctc
     2341 ctcttcttcc ttgaacccca tgccaccatc gcagctgcag ctgcccacac tgcccctagt
     2401 catggtggca ccctccgggg cacggctggg ccccttgccc cacttacagg cactcctcca
     2461 ggacaggcca catttcatgc accagctctc aacggtggat gcccacgccc ggacccctgt
     2521 gctgcaggtg caccccctgg agagcccagc catgatcagc ctcacaccac ccaccaccgc
     2581 cactggggtc ttctccctca aggcccggcc tggcctccca cctgggatca acgtggccag
     2641 cctggaatgg gtgtccaggg agccggcact gctctgcacc ttcccaaatc ccagtgcacc
     2701 caggaaggac agcacccttt cggctgtgcc ccagagctcc tacccactgc tggcaaatgg
     2761 tgtctgcaag tggcccggat gtgagaaggt cttcgaagag ccagaggact tcctcaagca
     2821 ctgccaggcg gaccatcttc tggatgagaa gggcagggca caatgtctcc tccagagaga
     2881 gatggtacag tctctggagc agcagctggt gctggagaag gagaagctga gtgccatgca
     2941 ggcccacctg gctgggaaaa tggcactgac caaggcttca tctgtggcat catccgacaa
     3001 gggctcctgc tgcatcgtag ctgctggcag ccaaggccct gtcgtcccag cctggtctgg
     3061 cccccgggag gcccctgaca gcctgtttgc tgtccggagg cacctgtggg gtagccatgg
     3121 aaacagcaca ttcccagagt tcctccacaa catggactac ttcaagttcc acaacatgcg
     3181 accccctttc acctacgcca cgctcatccg ctgggccatc ctggaggctc cagagaagca
     3241 gcggacactc aatgagatct accactggtt cacacgcatg tttgccttct tcagaaacca
     3301 tcctgccacc tggaagaacg ccatccgcca caacctgagt ctgcacaagt gctttgtgcg
     3361 ggtggagagc gagaaggggg ctgtgtggac cgtggatgag ctggagttcc gcaagaaacg
     3421 gagccagagg cccagcaggt gttccaaccc tacacctggc ccctgacctc aagatcaagg
     3481 aaaggaggat ggacgaacag gggccaaact ggtgggaggc agaggtggtg ggggcaggga
     3541 tgataggccc tggatgtgcc cacagggacc aagaagtgag gtttccactg tcttaagccg
     3601 aattcgatat caagcttatc gataccgtcg acctcgagat atctagaccc agctttcttg
     3661 tacaaagtgg ttcgatgacg gtatcgataa gcttgatatc gaattccgcc ccccccccta
     3721 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     3781 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     3841 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     3901 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     3961 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     4021 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     4081 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     4141 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     4201 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     4261 cacgatgata atatggccac aaccatgggt cttttctgca gtcaccgtcc tcgaggcacc
     4321 atgaacccag ccatcagcgt cgctctcctg ctctcagtct tgcaggtgtc ccgagggcag
     4381 aaggtgacca gcctgacagc ctgcctggtg aaccaaaacc ttcgcctgga ctgccgccat
     4441 gagaataaca ccaaggataa ctccatccag catgagttca gcctgacccg agagaagagg
     4501 aagcacgtgc tctcaggcac ccttgggata cccgagcaca cgtaccgctc ccgcgtcacc
     4561 ctctccaacc agccctatat caaggtcctt accctagcca acttcaccac caaggatgag
     4621 ggcgactact tttgtgagct tcgcgtctcg ggcgcgaatc ccatgagctc caataaaagt
     4681 atcagtgtgt atagagacaa gctggtcaag tgtggcggca taagcctgct ggttcagaac
     4741 acatcctgga tgctgctgct gctgctttcc ctctccctcc tccaagccct ggacttcatt
     4801 tctctgtgac gcgtagaatt cgataaaata aaagatttta tttagtctcc agaaaaaggg
     4861 gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa
     4921 ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg aacagagaga
     4981 cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     5041 caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag aaccatcaga
     5101 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
     5161 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
     5221 aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta cccgtgtatc
     5281 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
     5341 ctctgagtga ttgactaccc gtcagcgggg gtctttcatg ggtaacagtt tcttgaagtt
     5401 ggagaacaac attctgaggg taggagtcga atattaagta atcctgactc aattagccac
     5461 tgttttgaat ccacatactc caatactcct gaaatagttc attatggaca gcgcagaaag
     5521 agctggggag aattgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5581 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     5641 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     5701 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5761 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5821 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5881 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5941 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6001 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6061 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6121 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6181 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6241 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6301 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6361 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6421 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6481 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6541 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6601 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6661 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6721 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6781 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6841 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6901 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6961 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7021 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7081 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7141 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7201 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7261 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7321 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7381 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7441 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7501 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7561 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7621 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     7681 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     7741 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     7801 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     7861 ggcgggtgtc ggggctggct taactatgcg gcatc
//
