LOCUS       sequence-134171-        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloned region contains Acetobacter aceti 1023 purE(H59N) gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK340
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(604..620)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(604..620)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(617..639)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    628..644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(652..658,659..676,677..682))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    697..718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(835..852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1086..1105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcatttatc ttcggtaatg ggagagttgg ggacagaggc cgtctgcaat gcgcgccatg
       61 tttcaaggcg tgcggcaagg gccgggttat acagtgcaag aatggaggcg gccagcaagg
      121 cggcattttt ggcgccggat gccccaatag ccaaagtgcc cacaggcacg ccgccgggca
      181 tctgcacaat ggaaagcagg ctgtccatgc ctttaagagc gcgagattct actggaacac
      241 ccagcacggg caggcgtgtc catgccgcac acatacccgg cagatgtgca gctccaccgg
      301 ccccagcaat aatcacattc aggccgcgtt cggctgccgt acgcgcataa tccgccaacc
      361 ggtctggcgt acgatttgct gaaacaatga gggtttcatg cggaatttcc agctctgtca
      421 gcaatgcgtc tgcatggcgc atggtttccc agtctgactg gctgcccatg ataatgccca
      481 caacgggggc agaggctgct ttatcttcta aagcactgga agcagagggc aggggggcgg
      541 tttcgctcac ggtgggtatc tatccggttt tttaaagtct gtaacggagg aattcgtaat
      601 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      661 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      721 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      781 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1081 actataaaga taccaggcgt ttccccctgg aagc
//
