LOCUS       sequence-134174-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-MCS1-2A-MCS2
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..134
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        162..672
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     591..609
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(682..701)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tgcwwtagca gactcgttta gtgaccgtca gatcgcctgg agaattggct agcgaattcg
       61 tcgacaccgg ttccggagcc acgaacttct ctctgttaaa gcaagcagga gacgtggaag
      121 aaaaccccgg tcctaggctg cagacgcgtt gtacaggatc cggggttggg gttgcgcctt
      181 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
      241 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
      301 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
      361 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact
      421 agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct
      481 gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg
      541 cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca
      601 ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac
      661 ctctctcccc agcaattcac catgaccgag tacaagccca cggtgcgcct cgccacccgc
      721 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgcgactac ccgcacgcgc
      781 cacaccgtcg atcggacgca catcgagcgg gtcaccgagc tgcagaactc tctcmcgcgc
      841 gtcggctcga catcggcagg tgtgggtcgc gacgacgcgc gcgtgcgtct gaccacgcga
      901 agcgtcgagc gggggcgktc gcgratcggc cgscatggcg actgacggtt cgctgaccgc
      961 agccacagat gaagcctccg
//
