LOCUS       sequence-134181-        7757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Hemann MT, Bric A, Teruya-Feldstein J, Herbst A, Nilsson JA, 
            Cordon-Cardo C, Cleveland JL, Tansey WP, Lowe SW
  TITLE     Evasion of the p53 tumour surveillance network by tumour-derived MYC
            mutants.
  JOURNAL   Nature. 2005 Aug 11. 436(7052):807-11.
  PUBMED    16094360
REFERENCE   2  (bases 1 to 7757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2005 Aug 11. 436(7052):807-11."
COMMENT     Sequence Label: MSCV Myc T58A puro
FEATURES             Location/Qualifiers
     source          1..7757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             68..584
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    648..989
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1056..1472
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1402..1424
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1440..1456
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1505..1514
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1520..1546
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1526..1552
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             1565..1591
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1571..1597
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             1610..1636
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1616..1642
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             1658..2974
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="PLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPS
                     EDIWKKFELLPAPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTEL
                     LGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNP
                     ARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSL
                     LSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSP
                     SAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRK
                     CTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKAT
                     AYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     promoter        3000..3499
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3035..3058)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3432..3451
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3520..4119
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3520..3539)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4016..4036
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4219..4733
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    4895..4911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4919..4925,4926..4943,4944..4949))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4964..4985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5102..5119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5273..5861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5353..5372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6032..6823,6824..6892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6655..6674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6893..6997)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7065..7083
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7121..7143)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7243..7262
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7456..7478
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7606..7625
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tgaagcctat agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa
       61 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
      121 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga
      181 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
      241 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat
      301 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
      361 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
      421 ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgta
      481 ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg actcgctgat ccttgggagg
      541 gtctcctcag attgattgac tgcccacctc gggggtcttt catttggagg ttccaccgag
      601 atttggagac ccctgcccag ggaccaccga cccccccgcc gggaggtaag ctggccagcg
      661 gtcgtttcgt gtctgtctct gtctttgtgc gtgtttgtgc cggcatctaa tgtttgcgcc
      721 tgcgtctgta ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg
      781 agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt
      841 gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc
      901 tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt
      961 ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct
     1021 gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa
     1081 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg
     1141 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
     1201 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
     1261 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
     1321 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
     1381 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
     1441 tttatccagc cctcactcct tctctaggcg ccggaattag atcttcggcc agcgactagt
     1501 agaagccacc atggatagct atccttatga cgtgcctgac tatgccagcc tgatgctgat
     1561 gagctatcct tatgacgtgc ctgactatgc cagcctgatg ctgatgagct atccttatga
     1621 cgtgcctgac tatgccagcc tgatgctgat gtctagaccc ctcaacgtta gcttcaccaa
     1681 caggaactat gacctcgact acgactcggt gcagccgtat ttctactgcg acgaggagga
     1741 gaacttctac cagcagcagc agcagagcga gctgcagccc ccggcgccca gcgaggatat
     1801 ctggaagaaa ttcgagctgc tgcccgcgcc gcccctgtcc cctagccgcc gctccgggct
     1861 ctgctcgccc tcctacgttg cggtcacacc cttctccctt cggggagaca acgacggcgg
     1921 tggcgggagc ttctccacgg ccgaccagct ggagatggtg accgagctgc tgggaggaga
     1981 catggtgaac cagagtttca tctgcgaccc ggacgacgag accttcatca aaaacatcat
     2041 catccaggac tgtatgtgga gcggcttctc ggccgccgcc aagctcgtct cagagaagct
     2101 ggcctcctac caggctgcgc gcaaagacag cggcagcccg aaccccgccc gcggccacag
     2161 cgtctgctcc acctccagct tgtacctgca ggatctgagc gccgccgcct cagagtgcat
     2221 cgacccctcg gtggtcttcc cctaccctct caacgacagc agctcgccca agtcctgcgc
     2281 ctcgcaagac tccagcgcct tctctccgtc ctcggattct ctgctctcct cgacggagtc
     2341 ctccccgcag ggcagccccg agcccctggt gctccatgag gagacaccgc ccaccaccag
     2401 cagcgactct gaggaggaac aagaagatga ggaagaaatc gatgttgttt ctgtggaaaa
     2461 gaggcaggct cctggcaaaa ggtcagagtc tggatcacct tctgctggag gccacagcaa
     2521 acctcctcac agcccactgg tcctcaagag gtgccacgtc tccacacatc agcacaacta
     2581 cgcagcgcct ccctccactc ggaaggacta tcctgctgcc aagagggtca agttggacag
     2641 tgtcagagtc ctgagacaga tcagcaacaa ccgaaaatgc accagcccca ggtcctcgga
     2701 caccgaggag aatgtcaaga ggcgaacaca caacgtcttg gagcgccaga ggaggaacga
     2761 gctaaaacgg agcttttttg ccctgcgtga ccagatcccg gagttggaaa acaatgaaaa
     2821 ggcccccaag gtagttatcc ttaaaaaagc cacagcatac atcctgtccg tccaagcaga
     2881 ggagcaaaag ctcatttctg aagaggactt gttgcggaaa cgacgagaac agttgaaaca
     2941 caaacttgaa cagctacgga actcttgtgc gtaagtcgac ggatcctgag aattctaccg
     3001 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     3061 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     3121 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     3181 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     3241 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     3301 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     3361 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     3421 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     3481 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     3541 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     3601 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     3661 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     3721 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     3781 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     3841 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     3901 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     3961 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     4021 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     4081 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     4141 cgcccgaccg aaaggagcgc acgaccccat gcatcgataa aataaaagat tttatttagt
     4201 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     4261 acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt
     4321 taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     4381 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct
     4441 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     4501 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     4561 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg
     4621 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     4681 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catgggtaac
     4741 agtttcttga agttggagaa caacattctg agggtaggag tcgaatatta agtaatcctg
     4801 actcaattag ccactgtttt gaatccacat actccaatac tcctgaaata gttcattatg
     4861 gacagcgcag aaagagctgg ggagaattgt gaaattgtta tccgctcaca attccacaca
     4921 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4981 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5041 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5101 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5161 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5221 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5281 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5461 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5581 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5641 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5701 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5881 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5941 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6001 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6061 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6121 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6181 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6241 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6301 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6361 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6421 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6481 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6541 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6601 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6661 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6721 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6781 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6841 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6901 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6961 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7021 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     7081 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     7141 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     7201 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     7261 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     7321 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     7381 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     7441 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg
     7501 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
     7561 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
     7621 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
     7681 gaggcgatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac
     7741 tcaacaatat caccagc
//
