LOCUS       sequence-134182-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloned region contains Acetobacter aceti 1023 purE gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK324
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(600..616)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(600..616)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(613..635)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    624..640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(648..654,655..672,673..678))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    693..714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(831..848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttatcttcg gtaatgggag agttggggac agaggccgtc tgcaatgcgc gccatgtttc
       61 aaggcgtgcg gcaagggccg ggttatacag tgcaagaatg gaggcggcca gcaaggcggc
      121 atttttggcg ccggatgccc caatagccaa agtgcccaca ggcacgccgc cgggcatctg
      181 cacaatggaa agcaggctgt ccatgccttt aagagcgcga gattctactg gaacacccag
      241 cacgggcagg cgtgtccatg ccgcacacat acccggcaga tgtgcagctc caccggcccc
      301 agcaataatc acattcaggc cgcgttcggc tgccgtacgc gcataatccg ccaaccggtc
      361 tggcgtacga tgtgctgaaa caatgagggt ttcatgcgga atttccagct ctgtcagcaa
      421 tgcgtctgca tggcgcatgg tttcccagtc tgactggctg cccatgataa tgcccacaac
      481 gggggcagag gctgctttat cttctaaagc actggaagca gagggcaggg gggcggtttc
      541 gctcacggtg ggtatctatc cggtttttta aagtctgtaa cggaggaatt cgtaatcatg
      601 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      661 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      721 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      781 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
      841 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1021 cctgacgagc atcacaaaaa tcgacgctca agtca
//
