LOCUS       sequence-134189-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase, Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Kappock
  TITLE     Single Trp variants of Acetobacter aceti PurE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK526
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             651..673
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(704..723)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(705..723)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcaagc ttgtcgacgg agctcgaatt ctcatttatc ttcggtaatg ggagagttgg
      121 ggacagaggc cgtctgcaat gcgcgaaatg tttcaaggcg tgcggcaagg gccgggttat
      181 acagtgcaag aatggaggcg gccagcaagg cggcattttt ggcgccggat gccccaatag
      241 ccaaagtgcc cacaggcacg ccgccgggca tctgcacaat ggaaagcagg ctgtccatgc
      301 ctttaagagc gcgagattct actggaacac ccagcacggg caggcgtgta tatgccgcac
      361 acatacccgg cagatgtgca gctccaccgg ccccagcaat aatcacattc aggccgcgtt
      421 cggctgccgt acgcgcataa tccgccaacc ggtctggcgt acgatgtgct gaaacaatga
      481 gggtttcatg cggaatttcc agctctgtca gcaatgcgtc tgcatggcgc atggtttccc
      541 agtctgactg gctgcccatg ataatgccca caacgggggc agaggctgct ttatcttcta
      601 aagcactgga agcagagggc aggggggcgg tttcgctcat catatgtata tctccttctt
      661 aaagttaaac aaaattattt ctagagggaa accgttgtgg tctccctata gtgagtcgta
      721 ttaatttcgc gggatcgaga tctcgggcag cgttgggtcc tggccacggg tgcgcatgat
      781 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
      841 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
      901 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
      961 agcgcc
//
