LOCUS       sequence-134208-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  antisense probe for detection of human SNORD78 (C/D box snoRNA U78).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pET21d_SNORD78_probe
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             196..213
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(266..284)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      280..327
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      364..819
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(451..470)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     661..682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctagactcga gcggccgcca ctgtgctgga tatctgcaga attgcccttc acagggttct
       61 tcagtgttac ctttgtctac atgctcattt caggtcagac atttgatcaa catcattaca
      121 caaaccccaa gggcaattcc accacactgg actagtggat ccgagctcgg taccaagctt
      181 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
      241 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      301 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
      361 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      421 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      481 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      541 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      601 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      661 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      721 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      781 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta gtggcacttt tcggggaaat
      841 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      901 agacaataac cctgataaat gcttcaataa tattgannnn nnaagagtat gagtattcaa
      961 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctnn tttgctnnnc
     1021 ccnnaaacnn nggtgaaagt aaa
//
