LOCUS       sequence-134211-        1223 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  antisense probe for detection of human SNORD2 (C/D box snoRNA 
            snR39B).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pcDNA3.1_SNORD2 probe
FEATURES             Location/Qualifiers
     source          1..1223
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             196..237
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             247..264
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(287..304)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    293..517
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      563..989
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(650..669)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     860..881
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(998..1018)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     gap             1168..1185
                     /estimated_length=18
ORIGIN
        1 cttggtancg agctcggatc cactagtcca gtgtggtgga attgccctta taatcaagtg
       61 atcagcaatg agtattccct tcatttcagg tcagtcccga aagatgattg ccatcatttc
      121 actttaagga agggcaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag
      181 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
      241 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
      301 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
      361 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
      421 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
      481 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
      541 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      601 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      661 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      721 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
      781 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
      841 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
      901 tcggtctatt cttttgattt ataaggnttt tgccgatttc ggcctattgg nnaaaaaatg
      961 agctgattta acaaaattna acgcgaatta attctgtgga atgtgtgtca gttagggtgn
     1021 gnaagtcccc agntcccagc agnnananta tgnnngcntg catctnannn tcancancan
     1081 nnnnnnagnn cccagntccc nncagcanaa nnatncaagc angcatnnnn nnnntnngnn
     1141 nnnanncnnn nccnnannnc nnnnnncnnn nnnnnnnnnn nnnnntnncg cccnnngnnt
     1201 gaananannn tntnnnnnnn nng
//
