LOCUS       sequence-134218-        9708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human E2F7 minigene (exons 11,12,13/introns cassette) with inserted 
            SNORD115 binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pcDNA3.1_hE2F7mg_115mut
FEATURES             Location/Qualifiers
     source          1..9708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5205..5246
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5256..5273
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5296..5313)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5302..5526
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5572..6000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5659..5678)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5869..5890
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6009..6029)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6014..6344
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6195..6330
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6257..6276
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6411..7205
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6465..6484)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7075..7094
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7381..7502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7418..7437)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7472..7491
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7551..7567)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7551..7567)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7564..7586)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7575..7591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7599..7605,7606..7623,7624..7629))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7644..7665
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7782..7799)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7953..8541)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8033..8052)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8712..9503,9504..9572))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9335..9354
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9573..9677)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattgcc cttaaaccct
      961 cagattccac agaccttgcc tctcccaaga ctatgggtaa cagggcatct atacccctca
     1021 aagacattca tgtgaatggc caactccctg ctgcagaaga gatttcagga aaggcaacag
     1081 caaactctct tgtttcttct gagtggggaa atccttcaag aaatacagat gttgaaaagc
     1141 cttcaaaaga aaatgaaagc accaaagagc cttctttgct acaatatctt tgtgtgcagt
     1201 ctcctgcagg taagggatgt catcatgtct tagagtccct tgcggcagtc agatcaagaa
     1261 ttggattttt aaatttttgt ctgtgtcacc tgacccaaag tggcttattg atgtgcctga
     1321 gtcattttac tcttagcccc tttgttttct ccatctataa atgaagggag aagctgcttc
     1381 tactttcacc aacttcagga gtaatgggaa aataattgag gcaggtgatt caaaaaaaat
     1441 acattaaaca ttatttggtg aaaaagcacg gtaattcagc tgagtctagc attggtttag
     1501 tataatagtg tgaacctgtt ggccattgca tgcagctagt gttttgaggt cttgaacatc
     1561 tagaggtact gagtatatag aattgtggcc aagtgccagg tctggagtaa gagggcttga
     1621 catttaaaac cacttattat ctgcgtgaac ttaagtattc ttcatcctca ttggttaaac
     1681 agcagtaata atagcacctg cttcatatgg gtgctgtgaa aattgaatta aatttaataa
     1741 gataatacat ctaaagcact tagaataata gcacctggca cttagtaaga ctcaataaat
     1801 gctgttacta ttttgtatta aagccgaaat acagatgcca gattttaaaa atatgtactt
     1861 tgtacataaa gctgctcttg atgcaaagat atctaaattg attttcctca attccttgtt
     1921 ggcttaagcc taacctgcta gaacaggtta ggttagtatt tatgattatt cacttatcta
     1981 gaaaaaattc tttatttctt tagtaagtat cttttagtgc tttctctaag cactgagtat
     2041 tggggataca caaatgaaca aaaatagatt cagcccctgc tgaatgaaaa ttacagccca
     2101 gcacaggggt ttttaacttt ttctgtgcca tggacctttt ggcagtctgg tgaaggccat
     2161 gaattcctcc tcagaatgtt acggatgcat agaattaaat acatgggatt acaaaggaag
     2221 ccaattatat tgaatgcaac tagaaaaatc atttttaaaa ttactgatag taataaaaag
     2281 acctggcagc aagcccaata gtgtcaaaat ttccaagttg tgatgagctt aacagtatat
     2341 taagaaatct gtaataggtt taatgtaata tgcacatatc tgtactattt cctaatgaca
     2401 aagtcaaggt actgttaaaa ttgttaattt gttgcctgtg ataattgagg gaaataaaaa
     2461 ttttcagtta ggaacttata aagatatttt ggccaggcac agtggctcat gcctgtaatc
     2521 tcagcacttt gggaggctga ggtaggaaga tcacttgagc ccaggagttt gacaccagcc
     2581 gggcaacata ggaagaacct gtttctacaa aaaataaaaa aaaattagct gggtgtggtg
     2641 gcatgcttgt ggtcccagct actctgcaga ctgaaatggg acgactgctt gagcccagga
     2701 gggtgaggct acagtaagcc atgatcacac cactgcactc cagcctgggt aacagagcaa
     2761 gactgggtct caaaacaaaa caacaaaaaa taagtaaata aataaaaata aagatgtaat
     2821 ctttcccatc taagcttttt gccttctcag tttgggttca gtggaactca tgttaaaaac
     2881 ttcacaggca ccaactaaat gatcacacag ataaatgtgg gattacaatc ttggccacag
     2941 ctgtgaagga gcagggtgtg ggctgtttgg cctggtcagc tgggggaagg tctcatcatt
     3001 cattgattta ttgtggactc ccgtttgaaa gggccatgac aaatgtgagc ttgaatgata
     3061 tcctcatgat gacatatatt gattttcgcc tgttctgctt ttctttcagg attaaatggt
     3121 ttcaatgtac ttttatctgg cagtcaaacc ccccctactg tgggcccgtc ctcaggtcag
     3181 ctgccgtctt tcagtgtccc ttgcatggtc ttaccatctc cacctctggg cccttttcct
     3241 gttctctatt ctcctgcaat gccgggcccg gtttcttcta ctcttggtgc tctcccaaac
     3301 acaggacctg tgaatttcag cttgcctggc cttggatcaa tagcccagct tctcgtcggc
     3361 cccacagctg tggttaatcc aaagtcgtcc acactccctt ctgcagaccc tcagcttcag
     3421 agtcagccct cactaaacct aagtccagtg atgtcaaggt cacacagtgt cgtccaacaa
     3481 cctgagtccc ccgtttacgt gggacatcct agccagcgta atcctattga gcataggtga
     3541 ggcctcgtac gaagtttcct gaagacactg tcactctggg aaggggtatg cctgtgttta
     3601 gctttcattt tagccacatg actgccggac agaggcacta ggtcaggctc cacctggctt
     3661 ccgttgtaga cctagagtta tctgctcctt ttataaagca ctgagacatg aggtgagaga
     3721 tcctgtgctt taaagacaaa aatgtatgtt tttagttgtc attctgtttc ttataattag
     3781 agggacctgt tttaggcata gttcgggggg atttttgagg aatgctaaat atttttatta
     3841 ttttggtgat cttcaagctt ttatttcact aggtcaataa aagaagctac cctaaatttt
     3901 aattatacag ctataaaaac aatcaaagga aacagaagtc ttgatagtaa ctcagagatt
     3961 cttagcctgt cagtcatttc ttaagatgtg atgtttagag actgtgtgtg tgtataatgt
     4021 ctgtatgtat atacatctat aatacataga tgtatataca tctatacata cgcagaaaac
     4081 atacaaatat acacaaagat acgttatcaa tataagttaa tgagtctata tttgtccccc
     4141 aacctccatt tgtagttcaa gtattcgttg aattcctatt acacattagg ttgttcacag
     4201 tgattactta ctctcccaga caaagaatta tgtttagcca aaaatatttt agacaagtta
     4261 ttcagaggct caaaagtttg cgagacattg aaatgaacat gttaggtttg aaacaagatg
     4321 attatagcat aacatagaat tttctatcac catgtgccag gcaccgatct aggcagtgaa
     4381 gatagagcag tgaaccagtg aacaagataa tggtccctgc cattttatgg cacattaagg
     4441 gaataaatat ttaacattta acattagggg ataaatatta gcaagtaaag agaaagagcc
     4501 aacacacaaa ccatgttggc ctcacatgct ctaaagtgct ttacaattat cttatttaat
     4561 tcctacaacc ttgtgaagtc agtaatttta ccatcgccgt tggatggagg aagaaactga
     4621 gggacaggtt gcttaagtaa cttgtccaca gtggcagagc ccagtccctg gctccagata
     4681 ccaaagatac ctctttggta gactgccttt ctgcacccaa aacaacaaat atgaatttaa
     4741 ttttcagttg tggtaagcag gggagacaaa cggggtaagt gtgataagaa agcaaagaag
     4801 aaggaacgcc tgccttggga tcctaacaca caggtcaaga aagggctgcc tttctactca
     4861 gagatcagaa acaatatgac ttcacttctg ttttttcttt tttctttttt cagtcaccag
     4921 ttccagtgac ccccaagagc atccaacgca cacatcgtga gacgtttttc aagacacccg
     4981 gcagccttgg agaccctgtc ctgaagagaa gagaaaggaa ccagtcacga aacaccagct
     5041 cggcccagag gagactagaa atccccagcg gcggcgctga ctaacctgcc gctttgccag
     5101 gtgggggtgg gatcaaacgc cctgagagtc ccggatgtaa gggcaattct gcagatatcc
     5161 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
     5221 accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt
     5281 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     5341 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     5401 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     5461 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     5521 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     5581 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5641 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5701 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac
     5761 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5821 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5881 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5941 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6001 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca
     6061 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6121 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6181 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6241 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     6301 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     6361 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     6421 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     6481 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6541 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     6601 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     6661 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     6721 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     6781 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     6841 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     6901 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     6961 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     7021 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     7081 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     7141 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     7201 tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca
     7261 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     7321 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7381 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7441 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7501 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7561 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7621 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7681 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7741 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7801 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7861 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7921 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7981 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8041 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8101 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     8161 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8221 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8281 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8341 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     8401 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8461 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8521 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8581 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8641 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8701 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8761 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8821 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8881 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8941 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9001 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9061 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9121 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9181 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9241 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9301 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9361 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9421 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9481 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9541 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9601 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9661 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
