LOCUS       sequence-134219-        9738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag 
            on 5'-end.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9738)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 9738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pcDNA3.1_hE2F7mg_PP7-tag
FEATURES             Location/Qualifiers
     source          1..9738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5235..5276
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5286..5303
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5326..5343)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5332..5556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5602..6030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5689..5708)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5899..5920
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6039..6059)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6044..6374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6225..6360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6287..6306
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6441..7235
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6495..6514)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7105..7124
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7411..7532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7448..7467)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7502..7521
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7581..7597)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7581..7597)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7594..7616)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7605..7621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7629..7635,7636..7653,7654..7659))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7674..7695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7812..7829)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7983..8571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8063..8082)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8742..9533,9534..9602))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9365..9384
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9603..9707)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattgcc cttaagcttt
      961 aaggagttta tatggaaacc cttagacgtc aaaccctcag attccacaga ccttgcctct
     1021 cccaagacta tgggtaacag ggcatctata cccctcaaag acattcatgt gaatggccaa
     1081 ctccctgctg cagaagagat ttcaggaaag gcaacagcaa actctcttgt ttcttctgag
     1141 tggggaaatc cttcaagaaa tacagatgtt gaaaagcctt caaaagaaaa tgaaagcacc
     1201 aaagagcctt ctttgctaca atatctttgt gtgcagtctc ctgcaggtaa gggatgtcat
     1261 catgtcttag agtcccttgc ggcagtcaga tcaagaattg gatttttaaa tttttgtctg
     1321 tgtcacctga cccaaagtgg cttattgatg tgcctgagtc attttactct tagccccttt
     1381 gttttctcca tctataaatg aagggagaag ctgcttctac tttcaccaac ttcaggagta
     1441 atgggaaaat aattgaggca ggtgattcaa aaaaaataca ttaaacatta tttggtgaaa
     1501 aagcacggta attcagctga gtctagcatt ggtttagtat aatagtgtga acctgttggc
     1561 cattgcatgc agctagtgtt ttgaggtctt gaacatctag aggtactgag tatatagaat
     1621 tgtggccaag tgccaggtct ggagtaagag ggcttgacat ttaaaaccac ttattatctg
     1681 cgtgaactta agtattcttc atcctcattg gttaaacagc agtaataata gcacctgctt
     1741 catatgggtg ctgtgaaaat tgaattaaat ttaataagat aatacatcta aagcacttag
     1801 aataatagca cctggcactt agtaagactc aataaatgct gttactattt tgtattaaag
     1861 ccgaaataca gatgccagat tttaaaaata tgtactttgt acataaagct gctcttgatg
     1921 caaagatatc taaattgatt ttcctcaatt ccttgttggc ttaagcctaa cctgctagaa
     1981 caggttaggt tagtatttat gattattcac ttatctagaa aaaattcttt atttctttag
     2041 taagtatctt ttagtgcttt ctctaagcac tgagtattgg ggatacacaa atgaacaaaa
     2101 atagattcag cccctgctga atgaaaatta cagcccagca caggggtttt taactttttc
     2161 tgtgccatgg accttttggc agtctggtga aggccatgaa ttcctcctca gaatgttacg
     2221 gatgcataga attaaataca tgggattaca aaggaagcca attatattga atgcaactag
     2281 aaaaatcatt tttaaaatta ctgatagtaa taaaaagacc tggcagcaag cccaatagtg
     2341 tcaaaatttc caagttgtga tgagcttaac agtatattaa gaaatctgta ataggtttaa
     2401 tgtaatatgc acatatctgt actatttcct aatgacaaag tcaaggtact gttaaaattg
     2461 ttaatttgtt gcctgtgata attgagggaa ataaaaattt tcagttagga acttataaag
     2521 atattttggc caggcacagt ggctcatgcc tgtaatctca gcactttggg aggctgaggt
     2581 aggaagatca cttgagccca ggagtttgac accagccggg caacatagga agaacctgtt
     2641 tctacaaaaa ataaaaaaaa attagctggg tgtggtggca tgcttgtggt cccagctact
     2701 ctgcagactg aaatgggacg actgcttgag cccaggaggg tgaggctaca gtaagccatg
     2761 atcacaccac tgcactccag cctgggtaac agagcaagac tgggtctcaa aacaaaacaa
     2821 caaaaaataa gtaaataaat aaaaataaag atgtaatctt tcccatctaa gctttttgcc
     2881 ttctcagttt gggttcagtg gaactcatgt taaaaacttc acaggcacca actaaatgat
     2941 cacacagata aatgtgggat tacaatcttg gccacagctg tgaaggagca gggtgtgggc
     3001 tgtttggcct ggtcagctgg gggaaggtct catcattcat tgatttattg tggactcccg
     3061 tttgaaaggg ccatgacaaa tgtgagcttg aatgatatcc tcatgatgac atatattgat
     3121 tttcgcctgt tctgcttttc tttcaggatt aaatggtttc aatgtacttt tatctggcag
     3181 tcaaaccccc cctactgtgg gcccgtcctc aggtcagctg ccgtctttca gtgtcccttg
     3241 catggtctta ccatctccac ctctgggccc ttttcctgtt ctctattctc ctgcaatgcc
     3301 gggcccggtt tcttctactc ttggtgctct cccaaacaca ggacctgtga atttcagctt
     3361 gcctggcctt ggatcaatag cccagcttct cgtcggcccc acagctgtgg ttaatccaaa
     3421 gtcgtccaca ctcccttctg cagaccctca gcttcagagt cagccctcac taaacctaag
     3481 tccagtgatg tcaaggtcac acagtgtcgt ccaacaacct gagtcccccg tttacgtggg
     3541 acatccagtc tcagtagtaa aattacatca ggtgaggcct gaagtttcct gaagacactg
     3601 tcactctggg aaggggtatg cctgtgttta gctttcattt tagccacatg actgccggac
     3661 agaggcacta ggtcaggctc cacctggctt ccgttgtaga cctagagtta tctgctcctt
     3721 ttataaagca ctgagacatg aggtgagaga tcctgtgctt taaagacaaa aatgtatgtt
     3781 tttagttgtc attctgtttc ttataattag agggacctgt tttaggcata gttcgggggg
     3841 atttttgagg aatgctaaat atttttatta ttttggtgat cttcaagctt ttatttcact
     3901 aggtcaataa aagaagctac cctaaatttt aattatacag ctataaaaac aatcaaagga
     3961 aacagaagtc ttgatagtaa ctcagagatt cttagcctgt cagtcatttc ttaagatgtg
     4021 atgtttagag actgtgtgtg tgtataatgt ctgtatgtat atacatctat aatacataga
     4081 tgtatataca tctatacata cgcagaaaac atacaaatat acacaaagat acgttatcaa
     4141 tataagttaa tgagtctata tttgtccccc aacctccatt tgtagttcaa gtattcgttg
     4201 aattcctatt acacattagg ttgttcacag tgattactta ctctcccaga caaagaatta
     4261 tgtttagcca aaaatatttt agacaagtta ttcagaggct caaaagtttg cgagacattg
     4321 aaatgaacat gttaggtttg aaacaagatg attatagcat aacatagaat tttctatcac
     4381 catgtgccag gcaccgatct aggcagtgaa gatagagcag tgaaccagtg aacaagataa
     4441 tggtccctgc cattttatgg cacattaagg gaataaatat ttaacattta acattagggg
     4501 ataaatatta gcaagtaaag agaaagagcc aacacacaaa ccatgttggc ctcacatgct
     4561 ctaaagtgct ttacaattat cttatttaat tcctacaacc ttgtgaagtc agtaatttta
     4621 ccatcgccgt tggatggagg aagaaactga gggacaggtt gcttaagtaa cttgtccaca
     4681 gtggcagagc ccagtccctg gctccagata ccaaagatac ctctttggta gactgccttt
     4741 ctgcacccaa aacaacaaat atgaatttaa ttttcagttg tggtaagcag gggagacaaa
     4801 cggggtaagt gtgataagaa agcaaagaag aaggaacgcc tgccttggga tcctaacaca
     4861 caggtcaaga aagggctgcc tttctactca gagatcagaa acaatatgac ttcacttctg
     4921 ttttttcttt tttctttttt cagtcaccag ttccagtgac ccccaagagc atccaacgca
     4981 cacatcgtga gacgtttttc aagacacccg gcagccttgg agaccctgtc ctgaagagaa
     5041 gagaaaggaa ccagtcacga aacaccagct cggcccagag gagactagaa atccccagcg
     5101 gcggcgctga ctaacctgcc gctttgccag gtgggggtgg gatcaaacgc cctgagagtc
     5161 ccggatgtaa gggcaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
     5221 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     5281 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     5341 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5401 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5461 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5521 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5581 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5641 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5701 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     5761 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5821 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5881 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5941 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     6001 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     6061 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     6121 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6301 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6361 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     6421 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     6481 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6541 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6601 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6661 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6721 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6781 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6841 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6901 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6961 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     7021 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7081 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     7141 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     7201 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg
     7261 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     7321 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     7381 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     7441 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7501 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     7561 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7621 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7681 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7741 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7801 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7861 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7921 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7981 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8041 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8101 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8161 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8221 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8281 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8341 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8401 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     8461 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8521 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8581 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8641 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8701 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8761 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8821 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8881 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8941 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9001 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9061 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9121 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9181 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9241 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9301 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9361 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9421 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9481 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9541 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9601 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9661 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9721 aaagtgccac ctgacgtc
//
