LOCUS       sequence-134220-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression clone for human mutated SNORD27 (C/D box snoRNA U27).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pcDNA3.1_SNORD27h_2ntmut
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             754..795
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             805..822
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(845..862)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gcttggtacc gagctcggat ccactagtcc agtgtggtgg aattgccctt atgttcagcc
       61 cacaagagct tactggtgaa ggaatgggac aagacccatc tttatgcaaa gccagcgtta
      121 cagtaatgtt ccaggtaggt gtacatggtt tatgctctta cagaggagac cttgtagata
      181 accactccat gatgaacaca aaatgacaag catatggctg aactttcaag tgatgtctgc
      241 ttactactga gaagtggaac tttcaagtga tgtctgctta ctactgagaa gtgagaggta
      301 gataaccact ccatgatgaa cacaaaatga caagcatatg gctgaacttt caagtgatgt
      361 ctgcttacta ctgagaagtg aactttcaag tgatgtctgc ttactactga gaagtggaac
      421 tttcaagtga tgtctgctta ctactgagaa gtggaacttt caagtgatgt ctgcttacta
      481 ctgagaagtg gaactttcaa gtgatgtctg cttactactg agaagtggaa ctttcaagtg
      541 atgtctgctt actactgaga agtgagagag aggtcttaag gggtctttga atgactattt
      601 ttaggtacat aaaatgcttt cctctgttgt ctacagcatc tcataatcta tcctggggaa
      661 ttcagctgcc tcccagggtg aatacagaag ggcaattctg cagatatcca gcacagtggc
      721 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
      781 ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga
      841 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
      901 tccttgaccc tggaaggtgc cactcccact gtcctttcct a
//
