LOCUS       sequence-134225-        9713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human E2F7 minigene (exons 11,12,13/introns cassette) with mutated 
            snord27 binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pcDNA3.1_hE2F7mg_10ntmut
FEATURES             Location/Qualifiers
     source          1..9713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5210..5251
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5261..5278
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5301..5318)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5307..5531
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5577..6005
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5664..5683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5874..5895
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6014..6034)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6019..6349
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6200..6335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6262..6281
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6416..7210
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6470..6489)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7080..7099
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7386..7507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7423..7442)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7477..7496
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7556..7572)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7556..7572)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7569..7591)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7580..7596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7604..7610,7611..7628,7629..7634))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7649..7670
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7787..7804)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7958..8546)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8038..8057)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8717..9508,9509..9577))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9340..9359
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9578..9682)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattgcc cttaaaccct
      961 cagattccac agaccttgcc tctcccaaga ctatgggtaa cagggcatct atacccctca
     1021 aagacattca tgtgaatggc caactccctg ctgcagaaga gatttcagga aaggcaacag
     1081 caaactctct tgtttcttct gagtggggaa atccttcaag aaatacagat gttgaaaagc
     1141 cttcaaaaga aaatgaaagc accaaagagc cttctttgct acaatatctt tgtgtgcagt
     1201 ctcctgcagg taagggatgt catcatgtct tagagtccct tgcggcagtc agatcaagaa
     1261 ttggattttt aaatttttgt ctgtgtcacc tgacccaaag tggcttattg atgtgcctga
     1321 gtcattttac tcttagcccc tttgttttct ccatctataa atgaagggag aagctgcttc
     1381 tactttcacc aacttcagga gtaatgggaa aataattgag gcaggtgatt caaaaaaaat
     1441 acattaaaca ttatttggtg aaaaagcacg gtaattcagc tgagtctagc attggtttag
     1501 tataatagtg tgaacctgtt ggccattgca tgcagctagt gttttgaggt cttgaacatc
     1561 tagaggtact gagtatatag aattgtggcc aagtgccagg tctggagtaa gagggcttga
     1621 catttaaaac cacttattat ctgcgtgaac ttaagtattc ttcatcctca ttggttaaac
     1681 agcagtaata atagcacctg cttcatatgg gtgctgtgaa aattgaatta aatttaataa
     1741 gataatacat ctaaagcact tagaataata gcacctggca cttagtaaga ctcaataaat
     1801 gctgttacta ttttgtatta aagccgaaat acagatgcca gattttaaaa atatgtactt
     1861 tgtacataaa gctgctcttg atgcaaagat atctaaattg attttcctca attccttgtt
     1921 ggcttaagcc taacctgcta gaacaggtta ggttagtatt tatgattatt cacttatcta
     1981 gaaaaaattc tttatttctt tagtaagtat cttttagtgc tttctctaag cactgagtat
     2041 tggggataca caaatgaaca aaaatagatt cagcccctgc tgaatgaaaa ttacagccca
     2101 gcacaggggt ttttaacttt ttctgtgcca tggacctttt ggcagtctgg tgaaggccat
     2161 gaattcctcc tcagaatgtt acggatgcat agaattaaat acatgggatt acaaaggaag
     2221 ccaattatat tgaatgcaac tagaaaaatc atttttaaaa ttactgatag taataaaaag
     2281 acctggcagc aagcccaata gtgtcaaaat ttccaagttg tgatgagctt aacagtatat
     2341 taagaaatct gtaataggtt taatgtaata tgcacatatc tgtactattt cctaatgaca
     2401 aagtcaaggt actgttaaaa ttgttaattt gttgcctgtg ataattgagg gaaataaaaa
     2461 ttttcagtta ggaacttata aagatatttt ggccaggcac agtggctcat gcctgtaatc
     2521 tcagcacttt gggaggctga ggtaggaaga tcacttgagc ccaggagttt gacaccagcc
     2581 gggcaacata ggaagaacct gtttctacaa aaaataaaaa aaaattagct gggtgtggtg
     2641 gcatgcttgt ggtcccagct actctgcaga ctgaaatggg acgactgctt gagcccagga
     2701 gggtgaggct acagtaagcc atgatcacac cactgcactc cagcctgggt aacagagcaa
     2761 gactgggtct caaaacaaaa caacaaaaaa taagtaaata aataaaaata aagatgtaat
     2821 ctttcccatc taagcttttt gccttctcag tttgggttca gtggaactca tgttaaaaac
     2881 ttcacaggca ccaactaaat gatcacacag ataaatgtgg gattacaatc ttggccacag
     2941 ctgtgaagga gcagggtgtg ggctgtttgg cctggtcagc tgggggaagg tctcatcatt
     3001 cattgattta ttgtggactc ccgtttgaaa gggccatgac aaatgtgagc ttgaatgata
     3061 tcctcatgat gacatatatt gattttcgcc tgttctgctt ttctttcagg attaaatggt
     3121 ttcaatgtac ttttatctgg cagtcaaacc ccccctactg tgggcccgtc ctcaggtcag
     3181 ctgccgtctt tcagtgtccc ttgcatggtc ttaccatctc cacctctggg cccttttcct
     3241 gttctctatt ctcctgcaat gccgggcccg gtttcttcta ctcttggtgc tctcccaaac
     3301 acaggacctg tgaatttcag cttgcctggc cttggatcaa tagcccagct tctcgtcggc
     3361 cccacagctg tggttaatcc aaagtcgtcc acactccctt ctgcagaccc tcagcttcag
     3421 agtcagccct cactaaacct aagtccagtg atgtcaaggt cacacagtgt cgtccaacaa
     3481 cctgagtccc ccgtttacgt gggacatcgc tagccagcat cagacaacca catacatcag
     3541 gtgaggcctc gtacggaagt ttcctgaaga cactgtcact ctgggaaggg gtatgcctgt
     3601 gtttagcttt cattttagcc acatgactgc cggacagagg cactaggtca ggctccacct
     3661 ggcttccgtt gtagacctag agttatctgc tccttttata aagcactgag acatgaggtg
     3721 agagatcctg tgctttaaag acaaaaatgt atgtttttag ttgtcattct gtttcttata
     3781 attagaggga cctgttttag gcatagttcg gggggatttt tgaggaatgc taaatatttt
     3841 tattattttg gtgatcttca agcttttatt tcactaggtc aataaaagaa gctaccctaa
     3901 attttaatta tacagctata aaaacaatca aaggaaacag aagtcttgat agtaactcag
     3961 agattcttag cctgtcagtc atttcttaag atgtgatgtt tagagactgt gtgtgtgtat
     4021 aatgtctgta tgtatataca tctataatac atagatgtat atacatctat acatacgcag
     4081 aaaacataca aatatacaca aagatacgtt atcaatataa gttaatgagt ctatatttgt
     4141 cccccaacct ccatttgtag ttcaagtatt cgttgaattc ctattacaca ttaggttgtt
     4201 cacagtgatt acttactctc ccagacaaag aattatgttt agccaaaaat attttagaca
     4261 agttattcag aggctcaaaa gtttgcgaga cattgaaatg aacatgttag gtttgaaaca
     4321 agatgattat agcataacat agaattttct atcaccatgt gccaggcacc gatctaggca
     4381 gtgaagatag agcagtgaac cagtgaacaa gataatggtc cctgccattt tatggcacat
     4441 taagggaata aatatttaac atttaacatt aggggataaa tattagcaag taaagagaaa
     4501 gagccaacac acaaaccatg ttggcctcac atgctctaaa gtgctttaca attatcttat
     4561 ttaattccta caaccttgtg aagtcagtaa ttttaccatc gccgttggat ggaggaagaa
     4621 actgagggac aggttgctta agtaacttgt ccacagtggc agagcccagt ccctggctcc
     4681 agataccaaa gatacctctt tggtagactg cctttctgca cccaaaacaa caaatatgaa
     4741 tttaattttc agttgtggta agcaggggag acaaacgggg taagtgtgat aagaaagcaa
     4801 agaagaagga acgcctgcct tgggatccta acacacaggt caagaaaggg ctgcctttct
     4861 actcagagat cagaaacaat atgacttcac ttctgttttt tcttttttct tttttcagtc
     4921 accagttcca gtgaccccca agagcatcca acgcacacat cgtgagacgt ttttcaagac
     4981 acccggcagc cttggagacc ctgtcctgaa gagaagagaa aggaaccagt cacgaaacac
     5041 cagctcggcc cagaggagac tagaaatccc cagcggcggc gctgactaac ctgccgcttt
     5101 gccaggtggg ggtgggatca aacgccctga gagtcccgga tgtaagggca attctgcaga
     5161 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     5221 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     5281 agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5341 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5401 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5461 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     5521 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     5581 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5641 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5701 cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc
     5761 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5821 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5881 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5941 gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6001 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc
     6061 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6121 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6181 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6241 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6301 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6361 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     6421 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6481 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6541 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6601 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6661 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6721 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6781 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6841 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6901 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     6961 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7021 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7081 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7141 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7201 gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc
     7261 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     7321 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     7381 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7441 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7501 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     7561 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7621 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7681 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7741 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7801 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7861 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7921 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7981 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8041 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8101 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     8161 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8221 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8281 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8341 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     8401 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8461 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8521 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8581 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8641 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8701 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8761 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8821 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8881 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8941 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9001 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9061 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9121 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9181 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9241 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9301 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9361 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9421 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9481 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9541 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9601 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9661 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
