LOCUS       sequence-134238-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Ser38>Asp mutant (TdPurE2-S38D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Kappock
  TITLE     Serine mutants of Treponema denticola PurE2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK644
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             600..622
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(653..672)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(654..672)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattcg agctccgtcg acaagaaact gaaacgctga attcagcgtt
      121 tcagtttcga atcgtcttcg ataattttct gcgcattgga ttccatatag gatttgaccg
      181 aatctgcgat ttctttatcg tacagtgaaa aaatacgagc cgccaggagt gccgcatttt
      241 tcggttcaag aaccagtgcc ggactaatcc cgctcggcat ccgtaacgaa gaataaatgt
      301 cggcgccggc gaagctatcg cttggcggtg ggcaggcaat ggttgcacct ttgacgaagc
      361 catcaacaaa gcctgataaa gcgttactac gcccagcgat cgtgatgtac agtttaggac
      421 ggtccaaagc ttcatattct ttcagcatag acactacatg ctccgctgtt ttatgcgcgt
      481 cgccgatacg tatggcatac tcaataccaa aagttttcaa ttcagacgca attttctcgg
      541 cgtgacccat atctgaactg ctgcccataa ggataatcac cagcggacgc atatgtatat
      601 ctccttctta aagttaaaca aaattatttc tagagggaaa ccgttgtggt ctccctatag
      661 tgagtcgtat taatttcgcg ggatcgagat ctcgggcagc gttgggtcct ggccacgggt
      721 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
      781 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
      841 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
      901 gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct
      961 accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt
     1021 tctctggtcc cgccgcatcc ataccgccag t
//
