LOCUS       sequence-134254-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase, Trp34>Phe/Trp97>Tyr mutant (AaPurE1fyw).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Kappock
  TITLE     Single Trp variants of Acetobacter aceti PurE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK525
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             647..669
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(700..719)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(701..719)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattctca tttatcttcg gtaatgggag agttggggac
      121 agaggccgtc tgcaatgcgc gccatgtttc aaggcgtgcg gcaagggccg ggttatacag
      181 tgcaagaatg gaggcggcca gcaaggcggc atttttggcg ccggatgccc caatagccaa
      241 agtgcccaca ggcacgccgc cgggcatctg cacaatggaa agcaggctgt ccatgccttt
      301 aagagcgcga gattctactg gaacacccag cacgggcagg cgtgtatatg ccgcacacat
      361 acccggcaga tgtgcagctc caccggcccc agcaataatc acattcaggc cgcgttcggc
      421 tgccgtacgc gcataatccg ccaaccggtc tggcgtacga tgtgctgaaa caatgagggt
      481 ttcatgcgga atttccagct ctgtcagcaa tgcgtctgca tggcgcatgg tttcgaagtc
      541 tgactggctg cccatgataa tgcccacaac gggggcagag gctgctttat cttctaaagc
      601 actggaagca gagggcaggg gggcggtttc gctcatcata tgtatatctc cttcttaaag
      661 ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc cctatagtga gtcgtattaa
      721 tttcgcggga tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg
      781 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa
      841 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca
      901 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg
      961 ccctgcacca ttatgttccg gatctgcatc gcaggatgc
//
