LOCUS       sequence-134259-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two 
            C-terminal SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE
  TITLE     Enhancing homology-directed genome editing by catalytically active 
            and inactive CRISPR-Cas9 using asymmetric donor DNA.
  JOURNAL   Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
  PUBMED    26789497
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481."
COMMENT     Sequence Label: pCR1054
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aattatcaaa cttacgttga gtgattaact tggcgtttag aagttgtctc caatagtttt
       61 tcatcttttt gactacttct tcacttggaa cgttatccga tttaccacga tttttatcag
      121 aacgcgttaa gaccttattg tctattgaat cgtctttaag gaaactttgt ggaacaatgt
      181 gatcgacatc ataatcactt aaacgattaa tatctaattc ttggtccaca tacatgtctc
      241 ttccattttg gagataatag agatagagct tttcattttg caattgagta ttttcaacag
      301 gatgctcttt aagaatctga cttcctaatt ctttgatacc ttcttcgatt cgtttcatac
      361 gctctcgcga atttttctgg cccttttgag ttgtctgatt ttcacgtgcc atttcaataa
      421 cgatattttc tggcttatgc cgccccatta ctttgaccaa ttcatcaaca acttttacag
      481 tctgtaaaat acctttttta atagcagggc taccagctaa atttgcaata tgttcatgta
      541 aactatcgcc ttgtccagac acttgtgctt tttgaatgtc ttctttaaat gtcaaactat
      601 catcatggat cagctgcata aaattgcgat tggcaaaacc atctgatttc aaaaaatcta
      661 atattgtttt gccagattgc ttatccctaa taccattaat caattttcga gacaaacgt
//
