LOCUS       sequence-134266-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Ser38>Ala/Phe79>Trp mutant (TdPurE2-S38A,F79W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Kappock
  TITLE     Serine mutants of Treponema denticola PurE2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK654
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             590..607
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(660..678)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      674..721
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(845..864)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc gtccgctggt gattatcctt
       61 atgggcagca gttcagatat gggtcacgcc gagaaaattg cgtctgaatt gaaaactttt
      121 ggtattgagt atgccattcg catcggcgcc gcgcataaaa cagcggagca tgtagtgtct
      181 atgctgaaag aatatgaagc tttggaccgt cctaaactgt acatcacgat cgctgggcgt
      241 agtaacgctt tatcaggctt tgttgatggc tgggtcaaag gtgcaaccat tgcctgccca
      301 ccgccaagcg atagcttcgc cggcgccgac atttattctt cgttacggat gccgagcggg
      361 attagtccgg cactggttct tgaaccgaaa aatgcggcac tcctggcggc tcgtattttt
      421 tcactgtacg ataaagaaat cgcagattcg gtcaaatcct atatggaatc caatgcgcag
      481 aaaattatcg aagacgattc gaaactgaaa cgctgaattc agcgtttcag tttcttgtcg
      541 acggagctcg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca
      601 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      661 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      721 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      781 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      841 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      901 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacgg
//
