LOCUS       sequence-134275-        1132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli thioredoxin reductase (EcTrxB).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK647
FEATURES             Location/Qualifiers
     source          1..1132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1009..1026
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1079..1097)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgg gcacgaccaa acacagtaaa
       61 ctgcttatcc tgggttcagg cccggcggga tacaccgctg ctgtctacgc ggcgcgcgcc
      121 aacctgcaac ctgtgctgat taccggcatg gaaaaaggcg gccaactgac caccaccacg
      181 gaagtggaaa actggcctgg cgatccaaac gatctgaccg gtccgttatt aatggagcgc
      241 atgcacgaac atgccaccaa gtttgaaact gagatcattt ttgatcatat caacaaggtg
      301 gatctgcaaa accgtccgtt ccgtctgaat ggcgataacg gcgaatacac ttgcgacgcg
      361 ctgattattg ccaccggagc ttctgcacgc tatctcggcc tgccctctga agaagccttt
      421 aaaggccgtg gggtttctgc ttgtgcaacc tgcgacggtt tcttctatcg caaccagaaa
      481 gttgcggtca tcggcggcgg caataccgcg gttgaagagg cgctgtatct gtctaacatc
      541 gcttcggaag tgcatctgat tcaccgccgt gacggtttcc gcgcggaaaa aatcctcatt
      601 aagcgcctga tggataaagt ggagaacggc aacatcattc tgcacaccaa ccgtacgctg
      661 gaagaagtga ccggcgatca aatgggtgtc actggcgttc gtctgcgcga tacgcaaaac
      721 agcgataaca tcgagtcact cgacgttgcc ggtctgtttg ttgctatcgg tcacagcccg
      781 aatactgcga ttttcgaagg gcagctggaa ctggaaaacg gctacatcaa agtacagtcg
      841 ggtattcatg gtaatgccac ccagaccagc attcctggcg tctttgccgc aggcgacgtg
      901 atggatcaca tttatcgcca ggccattact tcggccggta caggctgcat ggcagcactt
      961 gatgcggaac gctacctcga tggtttagct gacgcaaaat aactcgagca ccaccaccac
     1021 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     1081 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gg
//
