LOCUS       sequence-134276-        1156 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces E. coli thioredoxin reductase (EcTrxB).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK647
FEATURES             Location/Qualifiers
     source          1..1156
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1026..1048
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1079..1098)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1080..1098)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agttattttg
       61 cgtcagctaa accatcgagg tagcgttccg catcaagtgc tgccatgcag cctgtaccgg
      121 ccgaagtaat ggcctggcga taaatgtgat ccatcacgtc gcctgcggca aagacgccag
      181 gaatgctggt ctgggtggca ttaccatgaa tacccgactg tactttgatg tagccgtttt
      241 ccagttccag ctgcccttcg aaaatcgcag tattcgggct gtgaccgata gcaacaaaca
      301 gaccggcaac gtcgagtgac tcgatgttat cgctgttttg cgtatcgcgc agacgaacgc
      361 cagtgacacc catttgatcg ccggtcactt cttccagcgt acggttggtg tgcagaatga
      421 tgttgccgtt ctccacttta tccatcaggc gcttaatgag gattttttcc gcgcggaaac
      481 cgtcacggcg gtgaatcaga tgcacttccg aagcgatgtt agacagatac agcgcctctt
      541 caaccgcggt attgccgccg ccgatgaccg caactttctg gttgcgatag aagaaaccgt
      601 cgcaggttgc acaagcagaa accccacggc ctttaaaggc ttcttcagag ggcaggccga
      661 gatagcgtgc agaagctccg gtggcaataa tcagcgcgtc gcaagtgtat tcgccgttat
      721 cgccattcag acggaacgga cggttttgca gatccacctt gttgatatga tcaaaaatga
      781 tctcagtttc aaacttggtg gcatgttcgt gcatgcgctc cattaataac ggaccggtca
      841 gatcgtttgg atcgccaggc cagttttcca cttccgtggt ggtggtcagt tggccgcctt
      901 tttccatgcc ggtaatcagc acaggttgca ggttggcgcg cgccgcgtag acagcagcgg
      961 tgtatcccgc cgggcctgaa cccaggataa gcagtttact gtgtttggtc gtgcccatat
     1021 gtatatctcc ttcttaaagt taaacaaaat tatttctaga gggaaaccgt tgtggtctcc
     1081 ctatagtgag tcgtattaat ttcgcgggat cgagatctcg ggcagcgttg ggtcctggcc
     1141 acgggtgcgc atgatc
//
