LOCUS       sequence-134282-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses two gRNAs targeting GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R
  TITLE     DECKO: Single-oligo, dual-CRISPR deletion of genomic elements 
            including long non-coding RNAs.
  JOURNAL   BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
  PUBMED    26493208
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-10-23"; volume: "16(1):846. doi"; pages: " 
            10.1186/s12864-015-2086-z"
COMMENT     Sequence Label: pDECKO_GFP
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        35..110
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        117..331
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     252..271
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        360..435
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    491..608
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aggacgaaac accggagctg gacggcgacg taaagtttta gagctagaaa tagcaagtta
       61 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgaac
      121 gctgacgtca tcaacccgct ccaaggaatc gcgggcccag tgtcactagg cgggaacacc
      181 cagcgcgcgt gcgccctggc aggaagatgg ctgtgaggga caggggagtg gcgccctgca
      241 atatttgcat gtcgctatgt gttctgggaa atcaccataa acgtgaaatg tctttggatt
      301 tgggagtctt ataagttctg tatgagacca ctctttcccc agaacacccc catcggcgag
      361 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      421 gcaccgagtc ggtgcttttt taagcttggc gtaactagat cttgagacaa atggcagtat
      481 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      541 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
      601 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg
      661 cccgggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
      721 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      781 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagag
//
