LOCUS       sequence-134283-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses two gRNAs targeting GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R
  TITLE     DECKO: Single-oligo, dual-CRISPR deletion of genomic elements 
            including long non-coding RNAs.
  JOURNAL   BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
  PUBMED    26493208
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-10-23"; volume: "16(1):846. doi"; pages: " 
            10.1186/s12864-015-2086-z"
COMMENT     Sequence Label: pDECKO_GFP
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    276..393
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(449..524)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(553..767)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(613..632)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta ggcaccggat
       61 caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg ctctgcccac
      121 tgacgggcac cggagccaat tcccactcct ttcaagacct agaaggtcca ttagctgcaa
      181 agattcctct ctgtttaaaa ctttatccat ctttgcaccc gggccccctc gagccggcgc
      241 caaagtggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt
      301 ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca
      361 ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtctc
      421 aagatctagt tacgccaagc ttaaaaaagc accgactcgg tgccactttt tcaagttgat
      481 aacggactag ccttatttta acttgctatt tctagctcta aaactcgccg atgggggtgt
      541 tctggggaaa gagtggtctc atacagaact tataagactc ccaaatccaa agacatttca
      601 cgtttatggt gatttcccag aacacatagc gacatgcaaa tattgcaggg cgccactccc
      661 ctgtccctca cagccatctt cctgccaggg cgcacgcgcg ctgggtgttc ccgcctagtg
      721 acactgggcc cgcgattcct tggagcgggt tgatgacgtc agcgttcaaa aaagcaccga
      781 ctcggtgcca ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag
      841 ctctaaa
//
