LOCUS       sequence-134284-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses two gRNAs targeting MALAT1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R
  TITLE     DECKO: Single-oligo, dual-CRISPR deletion of genomic elements 
            including long non-coding RNAs.
  JOURNAL   BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
  PUBMED    26493208
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-10-23"; volume: "16(1):846. doi"; pages: " 
            10.1186/s12864-015-2086-z"
COMMENT     Sequence Label: pDECKO_Malat1_A
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        34..109
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        116..330
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     251..270
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        359..434
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    490..607
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggacgaaaca ccggaaccgg tggggctgcg tcagttttag agctagaaat agcaagttaa
       61 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgaacg
      121 ctgacgtcat caacccgctc caaggaatcg cgggcccagt gtcactaggc gggaacaccc
      181 agcgcgcgtg cgccctggca ggaagatggc tgtgagggac aggggagtgg cgccctgcaa
      241 tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatgt ctttggattt
      301 gggagtctta taagttctgt atgagaccac tctttcccgg caggagaggc cagttgcggt
      361 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      421 caccgagtcg gtgctttttt aagcttggcg taactagatc ttgagacaaa tggcagtatt
      481 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
      541 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
      601 aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc tcgagggggc
      661 ccgggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
      721 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
      781 gcccacagtc cccgagaagt tggg
//
