LOCUS       sequence-134285-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses two gRNAs targeting MALAT1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R
  TITLE     DECKO: Single-oligo, dual-CRISPR deletion of genomic elements 
            including long non-coding RNAs.
  JOURNAL   BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
  PUBMED    26493208
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-10-23"; volume: "16(1):846. doi"; pages: " 
            10.1186/s12864-015-2086-z"
COMMENT     Sequence Label: pDECKO_Malat1_A
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    271..388
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(444..519)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(548..762)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(608..627)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac cggatcaatt
       61 gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccactgacg
      121 ggcaccggag ccaattccca ctcctttcaa gacctagaag gtccattagc tgcaaagatt
      181 cctctctgtt taaaacttta tccatctttg cacccgggcc ccctcgagcc ggcgccaaag
      241 tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
      301 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
      361 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt gtctcaagat
      421 ctagttacgc caagcttaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
      481 actagcctta ttttaacttg ctatttctag ctctaaaacc gcaactggcc tctcctgccg
      541 ggaaagagtg gtctcataca gaacttataa gactcccaaa tccaaagaca tttcacgttt
      601 atggtgattt cccagaacac atagcgacat gcaaatattg cagggcgcca ctcccctgtc
      661 cctcacagcc atcttcctgc cagggcgcac gcgcgctggg tgttcccgcc tagtgacact
      721 gggcccgcga ttccttggag cgggttgatg acgtcagcgt tcaaaaaagc accgactcgg
      781 tgccacttt
//
