LOCUS       sequence-134287-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses two gRNAs targeting MALAT1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R
  TITLE     DECKO: Single-oligo, dual-CRISPR deletion of genomic elements 
            including long non-coding RNAs.
  JOURNAL   BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
  PUBMED    26493208
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-10-23"; volume: "16(1):846. doi"; pages: " 
            10.1186/s12864-015-2086-z"
COMMENT     Sequence Label: pDECKO_Malat1_B
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    270..387
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(443..518)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(547..761)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(607..626)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc ggatcaattg
       61 ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccactgacgg
      121 gcaccggagc caattcccac tcctttcaag acctagaagg tccattagct gcaaagattc
      181 ctctctgttt aaaactttat ccatctttgc acccgggccc cctcgagccg gcgccaaagt
      241 ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt
      301 aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac
      361 cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg tctcaagatc
      421 tagttacgcc aagcttaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga
      481 ctagccttat tttaacttgc tatttctagc tctaaaactc aggcgagctg aggcttccgg
      541 gaaagagtgg tctcatacag aacttataag actcccaaat ccaaagacat ttcacgttta
      601 tggtgatttc ccagaacaca tagcgacatg caaatattgc agggcgccac tcccctgtcc
      661 ctcacagcca tcttcctgcc agggcgcacg cgcgctgggt gttcccgcct agtgacactg
      721 ggcccgcgat tccttggagc gggttgatga cgtcagcgtt caaaaaagca ccgactcggt
      781 gccacttttt caagttgata
//
