LOCUS       sequence-134291-        1111 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 thioredoxin reductase 1 with 
            Cys138>Ser mutant (AaTrxB1-C138S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 thioredoxin-associated genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK558
FEATURES             Location/Qualifiers
     source          1..1111
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             1044..1066
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcatc agctcaaacc agcaagatac cgttccgcat
      121 ctagcgcagc catacagcca gtcccagccg cagtgacggc ctggcggtat gttttgtcct
      181 gcacatcccc cgcggcaaag actccgggca cagatgtacg tgtggtgccg ggtgtggttt
      241 caatataccc ttcagcatcc agagtaagct ggttgcggaa gatgcctgtg ttaggggcgt
      301 gcccaatggc aataaatacg ccatcggtcg agacagtctg ttcgctatta tcctgcgtat
      361 tcttgagccg cacaccgcat acggtttctg gcgttccgtc ggccagaata tcttgcacca
      421 cgctgttcca gatcaccgaa accttcgggt tggcaaaaag ccggtcttgc agaatttttt
      481 cagcacgcag gctgtctcgg cggtgcacca aggtgacatg cgcggcatgg tgggtgagat
      541 acagggcctc ttcaaccgca gtattaccgc cgccgacaac cacaacgttc ttcccgcggt
      601 agaaaaagcc atcgcaggtg gcgctagcag aaacaccggc accttgcaga tgcttttcac
      661 ttggcaggcc aagccagcgt gcctgtgcgc ctgtggcaac aataacagca cgggccttgt
      721 aggttgtgcc ggaatcacct gttagcagaa aaggtgaacc cgctttgaaa ttaacagatg
      781 taataatgtc gtattcaatg cgtgtgccca cgttttgcgc ctgggctgcc atttgttcca
      841 tcagccacgg cccctgtatg gctgtggcaa aacccggata gttttcaaca tctgtagtaa
      901 tggtgagctg cccgccaggc tgaaggccag cgaccagaac gggagaaagg ttggcacgtg
      961 ccgcataaat agctgcggtg tatccggcag gtcccgcacc aataacgagc agatcagttg
     1021 taatggtttc agccatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg
     1081 gaaaccgttg tggtctccct atagtgagtc g
//
