LOCUS       sequence-134296-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses two gRNAs targeting MALAT1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R
  TITLE     DECKO: Single-oligo, dual-CRISPR deletion of genomic elements 
            including long non-coding RNAs.
  JOURNAL   BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
  PUBMED    26493208
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-10-23"; volume: "16(1):846. doi"; pages: " 
            10.1186/s12864-015-2086-z"
COMMENT     Sequence Label: pDECKO_Malat1_E
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    272..389
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(445..520)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(549..763)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(609..628)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 gtacacgaac atcactttcc cagtttaccc cgcgccacct tctctaggca ccggatcaat
       61 tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac
      121 gggcaccgga gccaattccc actcctttca agacctagaa ggtccattag ctgcaaagat
      181 tcctctctgt ttaaaacttt atccatcttt gcacccgggc cccctcgagc cggcgccaaa
      241 gtggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt
      301 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
      361 accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctcaaga
      421 tctagttacg ccaagcttaa aaaagcaccg actcggtgcc actttttcaa gttgataacg
      481 gactagcctt attttaactt gctatttcta gctctaaaac ttacgaaagt ccttcacatt
      541 gggaaagagt ggtctcatac agaacttata agactcccaa atccaaagac atttcacgtt
      601 tatggtgatt tcccagaaca catagcgaca tgcaaatatt gcagggcgcc actcccctgt
      661 ccctcacagc catcttcctg ccagggcgca cgcgcgctgg gtgttcccgc ctagtgacac
      721 tgggcccgcg attccttgga gcgggttgat gacgtcagcg ttcaaaaaag caccgactcg
      781 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct
      841 aa
//
