LOCUS       sequence-134297-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Synthetic gene corresponding to Synechocystis PCC6803 orf sll1489.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Kappock
  TITLE     Synechocystis PCC6803 CpmA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK588
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattca
       61 agcggcgtgg atctgggcac cgaaaatctg tactttcagt ctgtgtctgg cccggtcttc
      121 cagtttagtg caccgatgac gccggatagc ctgcaacagc tgctgacggc aatcgcaagc
      181 ggtcacatta gctctcaaga aggctttgaa cagctgaaac atctgagttt tcaggcgatc
      241 gatgacttcg ccaaagtgga tcaccagcgt caactgcgca ccggtttccc ggaagtggtt
      301 tggggcccgg gtaaaacgcc ggaacagatt gaacaaatta tccaggtcct ggcagtgcat
      361 aacccggtcg tgctggttac ccgtgtcgaa ccggaagtgg ctgatctgct gggcgaccgt
      421 attccgctgc tgcaatatta cccgcaggca cgcatctgcg ctctggttca aaccccgctg
      481 cagattaaat attcaggcac catcggtgtt ctgtcggccg gtacggcaga tctgccggtc
      541 gcagaagaag cggccattac ggccagcctg tgtggtttta aagttgaaaa actgtgggat
      601 gttggcgtcg cagggatcca tcgtctgctg tctcaccgcg aactgattca ggccatggac
      661 gtgctgatcg ttgtcgcagg catggaaggt gctctgccgt ccgtggttgc aggcctggtt
      721 gattgcccgg tgatcggtgt tccgaccagt gtcggctacg gcacgtcctt tggcggtgtg
      781 gcaccgctgc tgaccatgct gaatagctgt gctgtgggcg ttggtgtcgt gaacattgac
      841 aatggcttcg gtgcagctat gctggccggt cagatcctgc gtaccgttga ccgtctgcaa
      901 agtaaatcca cgatggcgaa tggcggc
//
