LOCUS       sequence-134307-       11002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of NP1 resistant to silencing by RNAi with 
            pLVTH-shNP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Figueiro-Silva J, Gruart A, Clayton KB, Podlesniy P, Abad MA, Gasull
            X, Delgado-Garcia JM, Trullas R
  TITLE     Neuronal pentraxin 1 negatively regulates excitatory synapse density
            and synaptic plasticity.
  JOURNAL   J Neurosci. 2015 Apr 8;35(14):5504-21. doi: 
            10.1523/JNEUROSCI.2548-14.2015.
  PUBMED    25855168
REFERENCE   2  (bases 1 to 11002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2548-14"; journalName: "J Neurosci"; date: 
            "2015-04-8- 8"; volume: "35"; issue: "14"; pages: "5504-21"
COMMENT     Sequence Label: pWPXL-resNP1
FEATURES             Location/Qualifiers
     source          1..11002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4832..5420
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4885..4905)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5303..5314)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5564..5597)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5617..5797
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5822..5840)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5823..5840)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6130..6149)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6363..6382)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6482..6504
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6542..6560)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6628..6732
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6733..6801,6802..7593)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6951..6970)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7764..8352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8253..8272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8506..8523
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8593..8613)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8598..8927
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8778..8913
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10061..10126
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10256..10276
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10701..10835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10738..10757)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10792..10811
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgaggttt aaactacggg atccaccatg ctggccggcc gcgccgcacg cacctgtgcg
     3541 ctgctcgccc tctgcctcct gggcagtcgg gcccaggatt tcgggccgac ccgcttcatc
     3601 tgcacttcgg tgccggtgga cgccgacatg tgtgccgcgt ccgtggccgc gggcggcgcg
     3661 gaggagcttc ggagcaatgt gctgcagctc cgggagaccg tgctgcagca gaaggagacc
     3721 atcctcagcc agaaggagac catcagggag ctgaccacca agcttggccg ctgcgagagc
     3781 cagagcaccc tggacgcggg tccgggcgag gccaggtcgg gcggcggccg caagcagcct
     3841 ggctcgggca agaatacgat gggcgacctg tcccggactc cggcctcgga gacgctcagc
     3901 caactcgggc aaactttgca atccctcaaa acccgactgg agaacctcga gcaatattcc
     3961 agactgaatt cttccagcca aaccaacagc ctcaaggatc tgctgcagag caagatcgat
     4021 gacctggagc ggcaggtgct gtctcgagtg aacactctgg aggagggaaa agggggcccc
     4081 aagaacgaca cagaggaaag ggccaagatc gagagcgcct tgacctccct acaccagcgg
     4141 atcagcgagc tggagaaagg tcagaaagac aatcgacccg gagacaagtt tcagctgaca
     4201 ttcccactgc ggaccaacta catgtatgcc aaggtgaaga agagcctgcc ggagatgtac
     4261 gccttcactg tgtgcatgtg gctcaagtcc agcgcggcac ctggagtggg cacgcccttc
     4321 tcctatgctg tacctgggca ggccaacgag ctggtcctca tcgagtgggg caacaacccc
     4381 atggagatac tcattaacga caaggtggcc aagctgccct ttgtaatcaa cgacggcaag
     4441 tggcaccaca tatgcgttac ctggaccacc cgggacgggg tctgggaggc ctatcaggat
     4501 ggcacccagg gaggcaatgg agagaacctg gcaccctatc accctatcaa accacagggt
     4561 gtgctggtgc taggccagga gcaggacact ctaggcggag ggtttgatgc cacccaagcg
     4621 tttgtgggtg agctagccca tttcaacatc tgggaccgca agctgacccc tggggaggtg
     4681 tacaacttgg ccacctgcag cagcaaggcc ctctctggca atgtcatcgc ctgggctgag
     4741 tcccagatcg agatctttgg aggagccacc aagtggacat tcgaggcttg ccgccagatc
     4801 aactgagaat tcccgggact agtcatatga taatcaacct ctggattaca aaatttgtga
     4861 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4921 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4981 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5041 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5101 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5161 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5221 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5281 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5341 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5401 cctttgggcc gcctccccgc atcggtacgt atggccaggt acctttaaga ccaatgactt
     5461 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5521 ttcactccca acgaagacaa gatgggatca attcaccatg ggaataactt cgtatagcat
     5581 acattatacg aagttatgct gctttttgct tgtactgggt ctctctggtt agaccagatc
     5641 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     5701 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     5761 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
     5821 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     5881 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     5941 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     6001 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     6061 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     6121 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     6181 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     6241 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     6301 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     6361 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     6421 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6481 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     6541 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     6601 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6661 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6721 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6781 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6841 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6901 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6961 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7021 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7081 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7141 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7201 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7261 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7321 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7381 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7441 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7501 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7561 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7621 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7681 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7741 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7801 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7861 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7921 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7981 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8041 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8101 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     8161 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8221 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8281 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8341 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8401 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8461 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8521 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     8581 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     8641 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8701 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     8761 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8821 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8881 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca
     8941 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     9001 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     9061 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     9121 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     9181 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     9241 ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     9301 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     9361 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
     9421 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     9481 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
     9541 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     9601 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
     9661 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     9721 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     9781 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     9841 tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg
     9901 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
     9961 aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac
    10021 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
    10081 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
    10141 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
    10201 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
    10261 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
    10321 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
    10381 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
    10441 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
    10501 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
    10561 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
    10621 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    10681 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    10741 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10801 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    10861 atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat
    10921 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
    10981 atatgtacta caaacttagt ag
//
