LOCUS       sequence-134311-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a Halo-labeled fusion of nuclease deficient Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Deng W, Shi X, Tjian R, Lionnet T, Singer RH
  TITLE     CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in 
            fixed cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):11870-5. doi: 
            10.1073/pnas.1515692112. Epub 2015 Aug 31.
  PUBMED    26324940
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1515692112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-22- 22"; volume: "112"; issue: "38"; pages: "11870-5"
COMMENT     Sequence Label: pET302-6His-dCas9-Halo
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaataatttt gtttaaactt taagaaggag atatactatg catcatcatc atcatcacgt
       61 ggataagaaa tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat
      121 cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca
      181 cagtatcaaa aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc
      241 gactcgtctc aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta
      301 tctacaggag attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact
      361 tgaagagtct tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa
      421 tatagtagat gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa
      481 attggtagat tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat
      541 gattaagttt cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt
      601 ggacaaacta tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat
      661 taacgcaagt ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg
      721 attagaaaat ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct
      781 cattgctttg tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga
      841 tgctaaatta cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca
      901 aattggagat caatatgctg atttgt
//
