LOCUS       sequence-134312-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a Halo-labeled fusion of nuclease deficient Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Deng W, Shi X, Tjian R, Lionnet T, Singer RH
  TITLE     CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in 
            fixed cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):11870-5. doi: 
            10.1073/pnas.1515692112. Epub 2015 Aug 31.
  PUBMED    26324940
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1515692112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-22- 22"; volume: "112"; issue: "38"; pages: "11870-5"
COMMENT     Sequence Label: pET302-6His-dCas9-Halo
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagttcactc aaacctccac gttcagcttt cgttaaatta tcaaacttac gttgagtgat
       61 taacttggcg tttagaagtt gtctccaata gtttttcatc tttttgacta cttcttcact
      121 tggaacgtta tccgatttac cacgattttt atcagaacgc gttaagacct tattgtctat
      181 tgaatcgtct ttaaggaaac tttgtggaac aatggcatcg acatcataat cacttaaacg
      241 attaatatct aattcttggt ccacatacat gtctcttcca ttttggagat aatagagata
      301 gagcttttca ttttgcaatt gagtattttc aacaggatgc tctttaagaa tctgacttcc
      361 taattctttg ataccttctt cgattcgttt catacgctct cgcgaatttt tctggccctt
      421 ttgagttgtc tgattttcac gtgccatttc aataacgata ttttctggct tatgccgccc
      481 cattactttg accaattcat caacaacttt tacagtctgt aaaatacctt ttttaatagc
      541 agggctacca gctaaatttg caatatgttc atgtaaacta tcgccttgtc cagacacttg
      601 tgctttttga atgtcttctt taaatgtcaa actatcatca tggatcagct gcataaaatt
      661 gcgattggca aaaccatctg atttcaaaaa atctaatatt gttttgccag attgcttatc
      721 cctaatacca ttaatcaatt ttcgagacaa acgtccccaa ccagtataac ggcgacgttt
      781 aagctgtttc atcaccttat catcaaagag gtgagcatat gttttaagtc tttcctcaat
      841 catctcccta tcttcaaata aggtcaatgt taaaacaata tcctctaaga tatcttcatt
      901 ttcttcatta tccaaaaaat ctttatcttt aataattttt agcaaatcat ggtaggtacc
      961 t
//
