LOCUS       sequence-134313-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a Halo-labeled fusion of nuclease deficient Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Deng W, Shi X, Tjian R, Lionnet T, Singer RH
  TITLE     CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in 
            fixed cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):11870-5. doi: 
            10.1073/pnas.1515692112. Epub 2015 Aug 31.
  PUBMED    26324940
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1515692112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-22- 22"; volume: "112"; issue: "38"; pages: "11870-5"
COMMENT     Sequence Label: pET302-6His-dCas9-Halo
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(78..965)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
ORIGIN
        1 tcagcttcct ttcgggcttt gttagcagcc ggatccgatt atacctaggc tcgaatatca
       61 tcgatctcga gctattaacc ggaaatctcc agagtagaca gccagcgcgc gatctcgctg
      121 ccgatcaggt ccgggttgtc ttcttgcagc agattcagac ccgggccgat gtccacagcc
      181 ttgcagttag gcaggctttt ggccaggcga gcggcttcgg ccggtgggat cagaacgcct
      241 ggggtgcccc agaacagcag cttcgggaca ggggactggt gcagccagtc catgtattct
      301 tcgaccagcg cgacgatgtt cgctggctca ccggcgattg gcagctcgtt tgggaagcgc
      361 cacagtggct cgcggtcaac aggattcagg aacggctcgc ggtaatggtc catctcgact
      421 tcagtcagcg ggcggacgac acccatcggc agcgtaccct cgataaaaac gttctgatcg
      481 atgatcagct tgcggccgac gtcggtggtg cggaaggcct ggaaggtctc gcgggcaaat
      541 tctggccatt cgtcccaggt cgggataggg cggatgaact ccataaatgc aatacctttg
      601 acgcgctctg gattgcgctt ggcccagtgg aaacccagag cggagcccca gtcgtgaatg
      661 accaggacga cctcttccag acccagggct tcgatgaagg catccatgaa gcggacgtgg
      721 tcgtcgaaga aataacccag gtctggtttg tcggatttgc ccataccgat caggtctgga
      781 gcaatgcagc gatgggtcgg tgcaacatgc gggatgatgt tgcgccacac gtaggaggag
      841 gtcgggttac cgtgcaggaa cagcacaggg gtgccatcgc gcggaccaac atcgacgtag
      901 tgcatgcgct cgcccaggac ttccacataa tgggggtcga atggaaagcc agtaccgatt
      961 tctgcagatc tggagccacc gtcacctcct agctgactca aatcaatgcg tgtttcataa
     1021 agaccagtga tggattgatg gataagagtg gcatctaaaa cttcttttgt agacgtatat
     1081 cgtttacgat ca
//
