LOCUS       sequence-134315-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pCP5b
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             460..765
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     644..663
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
       61 gcagaatgct taatgaatta caacagtact gcgatgagtg gcaggggggg cgtaaacgcc
      121 gcgtggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
      181 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg
      241 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
      301 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
      361 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
      421 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
      481 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
      541 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
      601 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
      661 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
      721 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt
      781 atacacagcc agtctgcagg tcgatacagt agaaattaca gaaactttat cacgtttagt
      841 aagtatagag gctgaaaatc cagatgaagc cgaacgactt gtaagagaaa agtataagag
      901 ttgtgaaatt gttcttgatg cagatgattt tcaggactat gacactagcg tatatgaata
      961 ggtagatgtt ttt
//
