LOCUS       sequence-134316-        1129 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Shaw RJ, Kosmatka M, Bardeesy N, Hurley RL, Witters LA, DePinho RA, 
            Cantley LC
  TITLE     The tumor suppressor LKB1 kinase directly activates AMP-activated 
            kinase and regulates apoptosis in response to energy stress.
  JOURNAL   Proc Natl Acad Sci U S A 2004 Mar 9;101(10):3329-35. Epub 2004 Feb 
            25.
  PUBMED    14985505
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "2004-03-9"; volume: "101(10)"; pages: 
            "3329-35. Epub 2004 Feb 25"
COMMENT     Sequence Label: pcDNA3-FLAG-LKB1
FEATURES             Location/Qualifiers
     source          1..1129
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             103..126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttagccacca tggactacaa agacgatgac
      121 gataaagcaa gggatccact agtaacggcc gccagtgtgc tggaattctc cagcatggag
      181 gtggtggacc cgcagcagct gggcatgttc acggagggcg agctgatgtc ggtgggtatg
      241 gacacgttca tccaccgcat cgactccacc gaggtcatct accagccgcg ccgcaagcgg
      301 gccaagctca tcggcaagta cctgatgggg gacctgctgg gggaaggctc ttacggcaag
      361 gtgaaggagg tgctggactc ggagacgctg tgcaggaggg ccgtcaagat cctcaagaag
      421 aagaagttgc gaaggatccc caacggggag gccaacgtga agaaggaaat tcaactactg
      481 aggaggttac ggcacaaaaa tgtcatccag ctggtggatg tgttatacaa cgaagagaag
      541 cagaaaatgt atatggtgat ggagtactgc gtgtgtggca tgcaggaaat gctggacagc
      601 gtgccggaga agcgtttccc agtgtgccag gcccacgggt acttctgtca gctgattgac
      661 ggcctggagt acctgcatag ccagggcatt gtgcacaagg acatcaagcc ggggaacctg
      721 ctgctcacca ccggtggcac cctcaaaatc tccgacctgg gcgtggccga ggcactgcac
      781 ccgttcgcgg cggacgacac ctgccggacc agccagggct ccccggcttt ccagccgccc
      841 gagattgcca acggcctgga caccttctcc ggcttcaagg tggacatctg gtcggctggg
      901 gtcaccctct acaacatcac cacgggtctg taccccttcg aaggggacaa catctacaag
      961 ttgtttgaga acatcgggaa ggggagctac gccatcccgg gcgactgtgg ccccccgctc
     1021 tctgacctgc tgaaagggat gcttgagtac gaaccggcca agaggttctc catccggcag
     1081 atccggcagc acagctggtt ccggaagaaa catcctccgg ctgaagcac
//
