LOCUS       sequence-134318-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses two gRNAs targeting the TFRC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Aparicio-Prat E, Arnan C, Sala I, Bosch N, Guigo R, Johnson R
  TITLE     DECKO: Single-oligo, dual-CRISPR deletion of genomic elements 
            including long non-coding RNAs.
  JOURNAL   BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
  PUBMED    26493208
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-10-23"; volume: "16(1):846. doi"; pages: " 
            10.1186/s12864-015-2086-z"
COMMENT     Sequence Label: pDECKO_TFRC_B
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    273..390
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(446..521)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(550..764)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(610..629)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 cagtacacga catcactttc ccagtttacc ccgcgccacc ttctctaggc accggatcaa
       61 ttgccgaccc ctccccccaa cttctcgggg actgtgggcg atgtgcgctc tgcccactga
      121 cgggcaccgg agccaattcc cactcctttc aagacctaga aggtccatta gctgcaaaga
      181 ttcctctctg tttaaaactt tatccatctt tgcacccggg ccccctcgag ccggcgccaa
      241 agtggatctc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt
      301 tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg
      361 taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat ttgtctcaag
      421 atctagttac gccaagctta aaaaagcacc gactcggtgc cactttttca agttgataac
      481 ggactagcct tattttaact tgctatttct agctctaaaa cagccgccac ccgatatccc
      541 ggggaaagag tggtctcata cagaacttat aagactccca aatccaaaga catttcacgt
      601 ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc cactcccctg
      661 tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg cctagtgaca
      721 ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcaaaaaa gcaccgactc
      781 ggtgc
//
