LOCUS       sequence-134344-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase 
            (UctC) with Thr2>Ala mutant (UctC-T2A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Mullins EA, Francois JA, Kappock TJ
  TITLE     A specialized citric acid cycle requiring succinyl-coenzyme A 
            (CoA):acetate CoA-transferase (AarC) confers acetic acid resistance 
            on the acidophile Acetobacter aceti.
  JOURNAL   J Bacteriol. 2008 Jul;190(14):4933-40. doi: 10.1128/JB.00405-08. 
            Epub 2008 May 23.
  PUBMED    18502856
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.00405-08"; journalName: "J Bacteriol"; date: "2008-07"; 
            volume: "190"; issue: "14"; pages: "4933-40"
COMMENT     Sequence Label: pJK360
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattca ggcgtcattt tgcctctggt gcggcaaatt ccttacgcaa
      121 cgccgcgccc tgctcatcca gcttaggggc gccgggccgt tcatgtgggt catcataccc
      181 actgattttc accgggttac cgggcatcat caccccgcca gatttaatca gcatattccg
      241 cgcggctgtt tgtggcagat ttgccgcctc tgccacgtcc agcaacggcc caacaggcac
      301 accggctttg tgaataacat ccagccaatg cgccgctggc tgtttggaaa gtgctttttc
      361 caatgcagct ttcagcgcat cattattggc cattctatca tgattttcga caaaacgcgg
      421 gtctgtcgcc agttcaggcg caccaatcac atcgcaaagc gccttaaaaa gatggtcatt
      481 cccacaacag atgacaaaag gcttgtcctg cgcctgaaac acatcaaaag gcgccatata
      541 aggatggcga ttgccaatac gcgcaggggc tttgcctgta gcagaccagc acatcagagc
      601 atgctgcaaa aatgccaagg taccatcaaa cattgaaaca tcaatgtgtg cccctttccc
      661 tgtgcgctca cgcgcataaa gcgcactggc aatgccgcag aacatgaaaa ccccaccgca
      721 cagatcagaa agcgatgtgc cccctacccg cgttggcgga ccatcgggaa agcccgtggc
      781 catcatgatg ccgctcatgg cctgcacaat agtatcgtag gcaggataat gtgcgagcgg
      841 gcctgtctga ccgaaccctg atgaagatgc ataaatcaac cgtggattga tctttgccag
      901 ttcctcataa ttgaaaccca aacggtccat cacgccgggg cggaagtttt ccgccagcac
      961 atcggcctta cgcaccattt ccagaaaaat cgctcgatcg ccctcatcct tgaggttaag
     1021 ga
//
