LOCUS       sequence-134346-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner
            for enChIP analysis to purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Fujita T, Yuno M, Fujii H
  TITLE     An enChIP system for the analysis of bacterial genome functions.
  JOURNAL   BMC Res Notes. 2018 Jun 14;11(1):387. doi: 
            10.1186/s13104-018-3486-3.
  PUBMED    29898790
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2018-06-14"; volume: "11(1):387. doi"; pages: " 
            10.1186/s13104-018-3486-3"
COMMENT     Sequence Label: 3xFLAG-dCas9/p-bacteria
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        76..131
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    112..130
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             149..160
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             170..235
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
       61 taattcctaa tttttgttga cactctatcg ttgatagagt tattttacca ctccctatca
      121 gtgatagaga aaagaattca aaagatctaa agaggagaaa ggatctatgg actacaaaga
      181 ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg acaagctcat
      241 ggataagaaa tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat
      301 cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca
      361 cagtatcaaa aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc
      421 gactcgtctc aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta
      481 tctacaggag attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact
      541 tgaagagtct tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa
      601 tatagtagat gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa
      661 attggtagat tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat
      721 gattaagttt cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt
      781 ggacaaacta tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat
      841 taacgcaagt ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg
      901 attagaaaat ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct
      961 cattgctttg tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga
     1021 tg
//
