LOCUS       sequence-134347-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express in mammalian cells for unnatural amino acid incorporation 
            into VAMP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Vreja IC, Nikic I, Gottfert F, Bates M, Krohnert K, Outeiro TF, Hell
            SW, Lemke EA, Rizzoli SO
  TITLE     Super-resolution Microscopy of Clickable Amino Acids Reveals the 
            Effects of Fluorescent Protein Tagging on Protein Assemblies.
  JOURNAL   ACS Nano. 2015 Nov 24;9(11):11034-41. doi: 10.1021/acsnano.5b04434. 
            Epub 2015 Oct 28.
  PUBMED    26498474
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.5b04434"; journalName: "ACS Nano"; date: 
            "2015-11-24- 24"; volume: "9"; issue: "11"; pages: "11034-41"
COMMENT     Sequence Label: pN1 VAMP2 R125TAG
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(544..747)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(573..593)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
       61 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctagagtcg cggccgggct
      121 tactacggta ccgtcgactg cagaattcga gtgctgaagt aaacgatgat gatgatgagg
      181 atgatggcgc agatcactcc caagatgatc atcatcttga ggtttttcca ccagtatttg
      241 cgcttgagct tggctgcact tgtttcaaac tgggaggccc ctgcctggag ggcatctgca
      301 cggtcatcca gctccgacaa cttctggtcc cgctccagga ccttgtccac attcaccctc
      361 atgatgtcca ccacctcatc cacctgggcc tgggtctgct gcagtctcct gttactagta
      421 agatttggag gaggtgcagg ggggccaccc tcgccggccg gggcggcagg cgggacggtg
      481 gcagcggtag ccgacataga tctgagtccg gtagcgctag cggatctgac ggttcactaa
      541 accagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
      601 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
      661 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
      721 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
      781 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
      841 accgtcattg acgtcaatag g
//
