LOCUS       sequence-134371-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression GIV/Girdin F1685A mutation in Mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Lin C, Ear J, Midde K, Lopez-Sanchez I, Aznar N, Garcia-Marcos M, 
            Kufareva I, Abagyan R, Ghosh P
  TITLE     Structural basis for activation of trimeric Gi proteins by multiple 
            growth factor receptors via GIV/Girdin.
  JOURNAL   Mol Biol Cell. 2014 Nov 5;25(22):3654-71. doi: 
            10.1091/mbc.E14-05-0978. Epub 2014 Sep 3.
  PUBMED    25187647
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-05-0978"; journalName: "Mol Biol Cell"; date: 
            "2014-11-5- 5"; volume: "25"; issue: "22"; pages: "3654-71"
COMMENT     Sequence Label: GIV-F1685A FLAG
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(194..259)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggttgtcttc ccaacttgcc ccttgctcca taccaccccc ctccacccca taatattata
       61 gaaggacacc tagtcagaca aaatgatgca acttaatttt attaggacaa ggctggtggg
      121 cactggagtg gcaacttcca gggccaggag aggcactggg gaggggtcac agggatgcca
      181 cccgggatca ctacttgtca tcgtcatcct tgtagtcgat gtcatgatct ttataatcac
      241 cgtcatggtc tttgtagtca gcccgggatc ctgaggagct ttgttgctcc ctagacctgc
      301 tttttgaatt tctgctttct tgtactttgt ccacattaga tgctgcagtg gtgttgctgt
      361 cagcagcagc tggtggtgaa tcaactgata taggcagtct actgtccttg gtcaaaaaat
      421 catggatgct tgtccttcgt gtagttcctt cggcagttga gatcacgctg cttgcacgag
      481 gtaaagttgc ataagggtta ctatcttttg attgtcgtga cagagaagat tcttttacta
      541 attttatttt tccttgagtg cctggtgtag gttttcccgc agaactaatg aagtaggtat
      601 cttcagtttt tcgaggacca ggtctcaaaa actcaggctt agttgtccgt ctatcataga
      661 agtcccctgg ggtttttcca cttactgacc tggccagacc acagctaact ggtttgtctt
      721 ttcccaaaaa gtcagaagag acagacaaac ttttcattac ttcatctaaa agattctctt
      781 gacttgagga ctttatctgt actgaggtaa gcttattgct ttcttccaaa gcctgttgta
      841 gagtaacaac ttcacttcca ggggaaccag ttttgatctt gtgatgtcga ctctccagtg
      901 tttcagatat tttgtgctgg agcactgggc ttgatctggt ctgtcttttc aagtactgga
      961 ttggactgct accactgctg gaccaagcct catggtcatg aagcaggcta aattctccac
     1021 tgctgtggct ttgtggcctg ctttggttat taactgcacc tgctttgact tcatgtagtg
     1081 aagca
//
