LOCUS       sequence-134373-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide synthetase (PurK) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK130
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattctta actggcagat tcttcatctt ccacagcggc
      121 cgttttggtg gccaatgggc ccaaggcatc ctgaataccc aaattgcctg gcagcgcacc
      181 gtaggggaac agaatattta catgccccat tttacggccc actctggctt ctgccttgcc
      241 atatagatgc ggaataaagc cctgcgtggc cataatctgc ggcagcagcg ccatatcttc
      301 cggcccgaac agatttttca tgattgcatc agaatggcgc acagcgtgcg gcaaaggcag
      361 ccccgcaact gcacggatat gcatgtcaaa ttggtcctgc gggcaggcat tcatggtcca
      421 atggccagaa ttatgggggc gcggggccat ttcattcacc agcaggttgc cggtgcaatc
      481 aacaaacatt tcaacgccca gaataccaac cagctccagc ttttctgcaa tggtggtggc
      541 tagttttatg gcctgcgtgg caatatctgg gtcaatgggg gcgggggcta atgttagatc
      601 cagaataccg ttccagtgcc ggttcagcgc cgggtcaaag caccttaccg tgccatctgc
      661 accacgcgcc accataacac taatctcgca agcaaaatcc accatacctt ctgcaaccag
      721 aggatgtggt gccagagcgt tgaaagcctc ttccagatca tggatgtgat gcacacggcg
      781 ctggcctttg ccatcatatc cattgcgagt ggttttgaga atcagcggca aacccacttt
      841 ttctgcggct tcgtgtagcg ttttgacatc ggtaacagga tgccatggcg cgagtggaat
      901 acccgcagaa gcaaaaaaag ttttttctgc caatcggtcc tgactgatgc gcagaatatg
      961 ccccgaagga tgaacagggc agtgtttggc taggc
//
