LOCUS       sequence-134380-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant 
            (AaPurE1fwf-Y154I).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Kappock
  TITLE     Single Trp variants of Acetobacter aceti PurE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK563
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             639..661
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(692..711)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(693..711)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
       61 gtcgacggag ctcgaattct catttatctt cggtaatggg agagttgggg acagaggccg
      121 tctgcaatgc gcgaaatgtt tcaaggcgtg cggcaagggc cgggttgatc agtgcaagaa
      181 tggaggcggc cagcaaggcg gcatttttgg cgccggatgc cccaatagcc aaagtgccca
      241 caggcacgcc gccgggcatc tgcacaatgg aaagcaggct gtccatgcct ttaagagcgc
      301 gagattctac tggaacaccc agcacgggca ggcgtgtcca tgccgcacac atacccggca
      361 gatgtgcagc tccaccggcc ccagcaataa tcacattcag gccgcgttcg gctgccgtac
      421 gcgcataatc cgccaaccgg tctggcgtac gatgtgctga aacaatgagg gtttcatgcg
      481 gaatttccag ctctgtcagc aatgcgtctg catggcgcat ggtttcgaag tctgactggc
      541 tgcccatgat aatgcccaca acgggggcag aggctgcttt atcttctaaa gcactggaag
      601 cagagggcag gggggcggtt tcgctcatca tatgtatatc tccttcttaa agttaaacaa
      661 aattatttct agagggaaac cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg
      721 gatcgagatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
      781 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
      841 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
      901 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
      961 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
     1021 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
     1081 taccgccagt tgtttaccct
//
