LOCUS       sequence-134402-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Production of Acetobacter aceti 1023 thioredoxin (AaTrx).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Starks CM, Francois JA, MacArthur KM, Heard BZ, Kappock TJ
  TITLE     Atomic-resolution crystal structure of thioredoxin from the 
            acidophilic bacterium Acetobacter aceti.
  JOURNAL   Protein Sci. 2007 Jan;16(1):92-8.
  PUBMED    17192591
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2007-01"; volume: "16(1)"; pages: "92-8"
COMMENT     Sequence Label: pJK295
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             424..446
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(477..496)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(478..496)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattctaat tactgagcgc tttctaccca tgccttcaac
      121 tggcttttgg gcagggcacc cactttttta tctataacct tgccatcacg caccagcata
      181 agggttggaa tgctacgcac ctgatacgca tttggagttt ccgggttgtc atcaatattt
      241 accttggcaa ccgttacctt gccggcaaat tccttgccaa tttctcccaa tgccgggcca
      301 atcattttgc atgggccgca ccattctgcc cagaaatcta ccagcacaag gccagaggcc
      361 ttgagaacat cttgatcaaa gctggaatcg ctgacagcaa gcgtatgttc actcatatgt
      421 atatctcctt cttaaagtta aacaaaatta tttctagagg gaaaccgttg tggtctccct
      481 atagtgagtc gtattaattt cgcgggatcg agatctcggg cagcgttggg tcctggccac
      541 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
      601 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
      661 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
      721 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
      781 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
      841 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
      901 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
      961 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa
     1021 aaaccgccct taacatggcc cgcttt
//
