LOCUS       sequence-134416-        9704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector for tet-inducible expression of EGFP 
            tagged human RHOA-T19N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Kong YP, Carrion B, Singh RK, Putnam AJ
  TITLE     Matrix identity and tractional forces influence indirect cardiac 
            reprogramming.
  JOURNAL   Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474.
  PUBMED    24326998
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-12-11"; volume: "3:3474. doi"; pages: " 
            10.1038/srep03474"
COMMENT     Sequence Label: tetO-FUW-eGFP-RHOA-T19N
FEATURES             Location/Qualifiers
     source          1..9704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(1780..1798,1799..1821,1822..1840,1841..1863,1864..1882,
                     1883..1905,1906..1924,1925..1947,1948..1966,1967..1989,
                     1990..2008,2009..2031,2032..2050)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2083..2121
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2118..2142
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2227..2229,2230..2232,2233..2943)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2227..2229,2230..2232,2233..2943)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2272..2293)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2533..2552)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2880..2901
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(2950..3003,3004..3006,3007..3531)
                     /codon_start=1
                     /product="dominant negative mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (T19N)
                     /translation="MAAIRKKLVIVGDGACGKNCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     misc_feature    3562..4150
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3615..3635)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4033..4044)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4153..4169)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4154..4170)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4675..4855
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4881..4898)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4887..5111
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5157..5585
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5244..5263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5454..5475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5594..5614)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5599..5928
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5779..5914
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5841..5860
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5976..6023
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6042..6416
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6546..6667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6583..6602)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6637..6656
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6716..6732)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6716..6732)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6729..6751)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6740..6756
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6764..6770,6771..6788,6789..6794))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6809..6830
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6947..6964)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7118..7706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7198..7217)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7877..8668,8669..8737))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8500..8519
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8738..8842)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8917..8936)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9108..9487
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9489..9687
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt tagatctcga gtttaccact ccctatcagt gatagagaaa
     1801 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     1861 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     1921 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     1981 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2041 gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg
     2101 gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     2161 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattc
     2221 tctagaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2281 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2341 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2401 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     2461 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2521 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2581 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2641 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     2701 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     2761 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2821 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     2881 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     2941 aaggaattca tggctgccat ccggaagaaa ctggtgattg ttggtgatgg agcctgtgga
     3001 aagaactgct tgctcatagt cttcagcaag gaccagttcc cagaggtgta tgtgcccaca
     3061 gtgtttgaga actatgtggc agatatcgag gtggatggaa agcaggtaga gttggctttg
     3121 tgggacacag ctgggcagga agattatgat cgcctgaggc ccctctccta cccagatacc
     3181 gatgttatac tgatgtgttt ttccatcgac agccctgata gtttagaaaa catcccagaa
     3241 aagtggaccc cagaagtcaa gcatttctgt cccaacgtgc ccatcatcct ggttgggaat
     3301 aagaaggatc ttcggaatga tgagcacaca aggcgggagc tagccaagat gaagcaggag
     3361 ccggtgaaac ctgaagaagg cagagatatg gcaaacagga ttggcgcttt tgggtacatg
     3421 gagtgttcag caaagaccaa agatggagtg agagaggttt ttgaaatggc tacgagagct
     3481 gctctgcaag ctagacgtgg gaagaaaaaa tctgggtgcc ttgtcttgtg aggatccgaa
     3541 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     3601 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3661 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3721 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3781 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3841 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3901 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     3961 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4021 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4081 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4141 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
     4201 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     4261 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     4321 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     4381 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     4441 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     4501 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     4561 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     4621 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     4681 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4741 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4801 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     4861 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4921 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4981 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5041 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     5101 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     5161 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5221 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5281 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5341 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5401 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5461 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5521 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5581 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5641 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5701 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5761 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5821 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     5881 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     5941 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     6001 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     6061 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     6121 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     6181 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     6241 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     6301 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     6361 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     6421 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     6481 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6541 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6601 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6661 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6721 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6781 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6841 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6901 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6961 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7021 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7081 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7141 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7201 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7261 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7321 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7381 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7441 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7501 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7561 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7621 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7681 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7741 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7801 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7861 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7921 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7981 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8041 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8101 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8161 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8221 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8281 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8341 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8401 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8461 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8521 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8581 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8641 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8701 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8761 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8821 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     8881 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     8941 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     9001 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     9061 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     9121 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     9181 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     9241 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     9301 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     9361 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     9421 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     9481 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     9541 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     9601 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     9661 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tact
//
