LOCUS       sequence-134428-        1159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-tagged mSin1 (isoform 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ebner M, Sinkovics B, Szczygiel M, Ribeiro DW, Yudushkin I
  TITLE     Localization of mTORC2 activity inside cells.
  JOURNAL   J Cell Biol. 2017 Jan 31. pii: jcb.201610060. doi: 
            10.1083/jcb.201610060.
  PUBMED    28143890
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 31. pii: jcb.201610060. doi: 10.1083/jcb.201610060."
COMMENT     Sequence Label: N1-mSin1.1-GFP
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(3..710,711..713))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     CDS             complement(join(3..710,711..713))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(42..63)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     391..410
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     650..671
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
       61 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
      121 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      181 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      241 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
      301 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      361 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      421 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      481 atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg
      541 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
      601 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      661 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccaaggtt
      721 gcgaccggtg gatcccgggc ccgcggctgc tgcccggatt tcttctcctt ctggaagctg
      781 aagctcgtac gtctgttcag ttttctttgt ttttgagcaa agtagtcagc ccgggcagtg
      841 ctagctcgcg attccaggat gtagttaacc ttgagcacaa tttcattgac ggtagcagcg
      901 tccgattcaa agtagaggtg tttatagtcg tgattgctta gatacgtgag tttaaatatt
      961 gcgtgactgg ggcttttctc ttcagcaagg tcacaggcac agagcaggtc ggaatcgatt
     1021 garatgggtt tctgcttaat ccaaaactta gtgctggctt tctgattcgt aacagggtct
     1081 atctctactt tgtctccaga gatacctagc tgtacgtcgg ttgkgaatcg cagtctgggg
     1141 atcatgctga ctttgaatg
//
